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ALLELE –SPECIFIC OLIGONUCLEOTIDE (ASO) HYBRIDISATION IN DETECTING THE SICKLE CELL MUTATION Introduction to ASO.

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Presentation on theme: "ALLELE –SPECIFIC OLIGONUCLEOTIDE (ASO) HYBRIDISATION IN DETECTING THE SICKLE CELL MUTATION Introduction to ASO."— Presentation transcript:

1 ALLELE –SPECIFIC OLIGONUCLEOTIDE (ASO) HYBRIDISATION IN DETECTING THE SICKLE CELL MUTATION Introduction to ASO

2 ALLELE –SPECIFIC OLIGONUCLEOTIDE (ASO) HYBRIDISATION IN DETECTING THE SICKLE CELL MUTATION *ctgactcctgaggagaagtct gtgcatctgactcctgAggagaagtctgcc catgtagactgaggacTcctcttcagacgg gtgcatctgactcctgTggagaagtctgcc catgtagactgaggacTcctcttcagacgg *ctgactcctgtggagaagtct  A allele  S allele  A probe  S probe ASO Hybridisation

3 *ctgactcctgaggagaagtct  A probe *ctgactcctgtggagaagtct  S probe HYBRIDISE A AA A S SS S A SA S A AA A S SS S A SA S A AA A S SS S A SA S A AA A S SS S A SA S DETECTION A. HYBRIDISATION WITH  A PROBEB. HYBRIDISATION WITH  S PROBE Hybridisation Technique

4 Further Reading Wikipedia ‘Allele specific oligonucleotide’ article. Available: http://en.wikipedia.org/wiki/Allele- specific_oligonucleotidehttp://en.wikipedia.org/wiki/Allele- specific_oligonucleotide Wu et al (1989) Allele-specific enzymatic amplification of beta-globin genomic DNA for diagnosis of sickle cell anemia. Available: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2 86997/ http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2 86997/

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