1. Chapter 7 Membrane Structure and Function

2. Overview: Life at the Edge The plasma membrane is the boundary that separates the living cell from its surroundings The plasma membrane exhibits selective permeability, allowing some substances to cross it more easily than others Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

3. Concept 7.1: Cellular membranes are fluid mosaics of lipids and proteins Phospholipids are the most abundant lipid in the plasma membrane Phospholipids are amphipathic molecules, containing hydrophobic and hydrophilic regions The fluid mosaic model states that a membrane is a fluid structure with a “mosaic” of various proteins embedded in it Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

4. Membrane Models: Scientific Inquiry Membranes have been chemically analyzed and found to be made of proteins and lipids Scientists studying the plasma membrane reasoned that it must be a phospholipid bilayer Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

5. Fig. 7-2 Hydrophilic head WATER Hydrophobic tail WATER

6. In 1935, Hugh Davson and James Danielli proposed a sandwich model in which the phospholipid bilayer lies between two layers of globular proteins Later studies found problems with this model, particularly the placement of membrane proteins, which have hydrophilic and hydrophobic regions In 1972, J. Singer and G. Nicolson proposed that the membrane is a mosaic of proteins dispersed within the bilayer, with only the hydrophilic regions exposed to water Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

7. Fig. 7-3 Phospholipid bilayer Hydrophobic regions of protein Hydrophilic regions of protein

8. Freeze-fracture studies of the plasma membrane supported the fluid mosaic model Freeze-fracture is a specialized preparation technique that splits a membrane along the middle of the phospholipid bilayer Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

9. Fig. 7-4 TECHNIQUE Extracellular layer Knife Proteins Inside of extracellular layer RESULTS Inside of cytoplasmic layer Cytoplasmic layer Plasma membrane

10. The Fluidity of Membranes Phospholipids in the plasma membrane can move within the bilayer Most of the lipids, and some proteins, drift laterally Rarely does a molecule flip-flop transversely across the membrane Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

11. Fig. 7-5 Lateral movement (~10 7 times per second) Flip-flop (~ once per month) (a) Movement of phospholipids (b) Membrane fluidity Fluid Viscous Unsaturated hydrocarbon tails with kinks Saturated hydro- carbon tails (c) Cholesterol within the animal cell membrane Cholesterol

12. Fig. 7-5a (a) Movement of phospholipids Lateral movement (  10 7 times per second) Flip-flop (  once per month)

13. Fig. 7-6 RESULTS Membrane proteins Mouse cell Human cell Hybrid cell Mixed proteins after 1 hour

14. As temperatures cool, membranes switch from a fluid state to a solid state The temperature at which a membrane solidifies depends on the types of lipids Membranes rich in unsaturated fatty acids are more fluid that those rich in saturated fatty acids Membranes must be fluid to work properly; they are usually about as fluid as salad oil Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

15. Fig. 7-5b (b) Membrane fluidity Fluid Unsaturated hydrocarbon tails with kinks Viscous Saturated hydro- carbon tails

16. The steroid cholesterol has different effects on membrane fluidity at different temperatures At warm temperatures (such as 37°C), cholesterol restrains movement of phospholipids At cool temperatures, it maintains fluidity by preventing tight packing Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

17. Fig. 7-5c Cholesterol (c) Cholesterol within the animal cell membrane

18. Membrane Proteins and Their Functions A membrane is a collage of different proteins embedded in the fluid matrix of the lipid bilayer Proteins determine most of the membrane’s specific functions Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

19. Fig. 7-7 Fibers of extracellular matrix (ECM) Glyco- protein Microfilaments of cytoskeleton Cholesterol Peripheral proteins Integral protein CYTOPLASMIC SIDE OF MEMBRANE Glycolipid EXTRACELLULAR SIDE OF MEMBRANE Carbohydrate

20. Peripheral proteins are bound to the surface of the membrane Integral proteins penetrate the hydrophobic core Integral proteins that span the membrane are called transmembrane proteins The hydrophobic regions of an integral protein consist of one or more stretches of nonpolar amino acids, often coiled into alpha helices Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

21. Fig. 7-8 N-terminus C-terminus  Helix CYTOPLASMIC SIDE EXTRACELLULAR SIDE

22. Six major functions of membrane proteins: – Transport – Enzymatic activity – Signal transduction – Cell-cell recognition – Intercellular joining – Attachment to the cytoskeleton and extracellular matrix (ECM) Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

23. Fig. 7-9 (a) Transport ATP (b) Enzymatic activity Enzymes (c) Signal transduction Signal transduction Signaling molecule Receptor (d) Cell-cell recognition Glyco- protein (e) Intercellular joining (f) Attachment to the cytoskeleton and extracellular matrix (ECM)

24. The Role of Membrane Carbohydrates in Cell- Cell Recognition Cells recognize each other by binding to surface molecules, often carbohydrates, on the plasma membrane Membrane carbohydrates may be covalently bonded to lipids (forming glycolipids) or more commonly to proteins (forming glycoproteins) Carbohydrates on the external side of the plasma membrane vary among species, individuals, and even cell types in an individual Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

25. Synthesis and Sidedness of Membranes Membranes have distinct inside and outside faces The asymmetrical distribution of proteins, lipids, and associated carbohydrates in the plasma membrane is determined when the membrane is built by the ER and Golgi apparatus Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

26. Fig. 7-10 ER 1 Transmembrane glycoproteins Secretory protein Glycolipid 2 Golgi apparatus Vesicle 3 4 Secreted protein Transmembrane glycoprotein Plasma membrane: Cytoplasmic face Extracellular face Membrane glycolipid

27. Selectively permeable- allows certain substances to pass through By 2 ways: active or passive transport Passive- downhill Active- uphill (needs energy)

28. Passive: downhill reaction Simple diffusion Osmosis Facilitated diffusion Filtration Active: uphill reaction, needs ATP Exocytosis Endocytosis - Pinocytosis - Phagocytosis

29. No Barrier: Substances “spread out” High concentration to low concentration e.g.: Red dye placed in glass of water

30. Substances diffuse High concentration to low concentration Pores in membrane must be large “Down the concentration gradient” Dynamic equilibrium, equal rates in both directions Biological membrane:

31. Carrier proteins: Bind specific molecule & change shape Pass molecule through middle of protein

32. Osmosis- diffusion of a water through a semi- permeable membrane Moves down concentration gradient e.g., Two sugar solutions of different concentrations separated by porous membrane which lets water through but not sugar What will happen?

33. More concentrated to less concentrated Until concentration same on both sides: isotonic

34. Concentration of solute less: solution is hypotonic. Concentration of solute greater: solution is hypertonic.

35. Animal cells No cell walls Isotonic environment: Influx of water equals the efflux of water No change in cell shape

36. Hypotonic solution: Water enters cell Bursts, or lyses Hypertonic solution: Water leaves cell Shriveled, or crenate

38. Glomerular filtration

39. Passive transport & facilitated diffusion do NOT require ATP

40. DOES require the input of ATP Transport proteins AGAINST concentration gradient outside cell inside cell

42. ATP  ADP + P i + Energy

46. mucus http://www.1lecture.com/Physiology/Endocytosis%20and%20Exocytosis/index.html Goblet cell

48. Nerve Axon at Rest Na + /K + Pump http://www.1lecture.com/Biochemistry/cotransport/

49. H + Pump in a Plant

50. Ion channels - Voltage gated - Chemically gated - Mechanically gated Porins -Larger -Less specific Aquaporins - water Channel Proteins

51. Channel Proteins: Ion Channels

52. Channel Proteins: Porins MAC Barrel shaped protein

53. Channel Proteins: Aquaporins H2OH2O

54. Membrane Permeability Cell membrane: selectively permeable 4 factors that determine permeability 1. lipid solubility 2. molecular size 3. polarity 4.charge

55. Lipid solubility Most important factor Hydrophobic molecules Passively diffuse Hydrocarbons, carbon dioxide, & oxygen

56. Molecular Size and Polarity Larger molecules, less permeable Lower kinetic energy Small pore sizes in the membrane Polar molecules hydrophilic, less permeable Very small, polar uncharged (water) molecules can diffuse - + Molecular Size Polarity

57. Charge Charged molecules hydrophilic, less permeable Surrounded by coat of water (hydration shell), increases the size

58. You should now be able to: 1.Define the following terms: amphipathic molecules, aquaporins, diffusion 2.Explain how membrane fluidity is influenced by temperature and membrane composition 3.Distinguish between the following pairs or sets of terms: peripheral and integral membrane proteins; channel and carrier proteins; osmosis, facilitated diffusion, and active transport; hypertonic, hypotonic, and isotonic solutions Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

59. 4.Explain how transport proteins facilitate diffusion 5.Explain how an electrogenic pump creates voltage across a membrane, and name two electrogenic pumps 6.Explain how large molecules are transported across a cell membrane Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings