1. Set up Cornell Notes on pg. 9
Topic: 8.5 Translation
Essential Question:
Explain the connection between a codon and an amino acid.
Suppose a tRNA molecule had the anticodon AGU. What amino acid would it carry?
Don’t forget to add it to your T.O.Contents!
8.5 Translation
2.1 Atoms, Ions, and Molecules
Explain the connection between a codon and an amino acid.
Suppose a tRNA molecule had the anticodon AGU. What amino acid would it carry?
KEY CONCEPT Translation converts an mRNA message into a polypeptide, or protein.

2. KEY CONCEPT Translation converts an mRNA message into a polypeptide, or protein.

3. REVIEW:
Transcription makes three types of RNA.
Messenger RNA (mRNA) which was transcribed from DNA, carries the message that will be translated to form a protein.
Ribosomal RNA (rRNA) forms part of ribosomes where proteins are made.
Transfer RNA (tRNA) brings amino acids from the cytoplasm to a ribosome.

4. What do you think of when you hear the word translation?
We usually think of translating one language into another
Hello=Hola in Spanish
Are the “words” exactly the same?
But, do they mean the same thing? Do they have the same message?

5. Translation is a process that converts a message from one “language” into another.
Hey, I know a joke! A squirrel walks up to a tree and says, "I forgot to store acorns for the winter and now I am dead." Ha! It is funny because the squirrel gets dead.

6. Review: Protein Synthesis Video
Replication 1m40s
Transcription 2m 50s
Translation 4m 47s

7. Translation converts mRNA messages into polypeptides which make proteins.
mRNA can be translated into 20 different amino acids

8. How can just four nucleotides (A, U, C, and G) be translated into so many different amino acids?
The same way 26 letters of the alphabet can be translated into so many words.
ape
pea
The four letters of RNA are put together in different combinations to form many different “words”
A U C G

9. These “words” are called codons
A codon is a sequence of three nucleotides that codes for an amino acid.
codon for
methionine (Met)
leucine (Leu)

10. Please draw and label this picture of a segment of mRNA (pg. 8)
codon

11. THECATATETHERAT SISATETHEPIE HISDOGWASHOT HERFATCATSAT
Separate this frame into “codons”: (top of pg. 8)
THECATATETHERAT
SISATETHEPIE
HISDOGWASHOT
HERFATCATSAT

12. THECATATETHERAT THE CAT ATE THE RAT SISATETHEPIE SIS ATE THE PIE
Separate this frame into “codons”: (top of pg. 8)
THECATATETHERAT
THE CAT ATE THE RAT
SISATETHEPIE
SIS ATE THE PIE
HISDOGWASHOT
HIS DOG WAS HOT
HERFATCATSAT
HER FAT CAT SAT

13. The genetic code matches each codon to its amino acid or function.
1st two nucleotides are the most important

14. Tell me what these genetic codes code for (pg. 8)
Codon
Amino Acid
GCC
AAA
AGC
UAG
AUA
AAU
GCA
CCG
UCA
AGG

15. GCC- alanine (Ala)
AAA- Lysine (Lys)
AGC- Serine (Ser)
UAG- stop codon
AUA- Isoleucine (Iso)
AAU- Asparagine (Asp)
GCA- Alanine (Ala)
CCG- Proline (Pro)
UCA- Serine (Ser)
AGG- Arginine (Arg)

16. AUG UAG UAA UGA The Start codon codes for the start of a gene segment
Three stop codons code for the end of a gene segment
UAG
UAA
UGA

17. A change in the order in which codons are read changes the resulting protein.
Please use your genetic code sheet to identify the amino acid for each codon
Label each reading frame

18. A change in the order in which codons are read changes the resulting protein.

19. RANTHEFATCATSATONAHATHISSISWASMAD

20. Regardless of the organism, codons code for the same amino acid.

21. Get 3 colors
Color mRNA w/codons
Color tRNA (body/anticodon)
Color Amino Acid

22. Anticodon: AUG Codon: UAC
An anticodon is a set of three nucleotides that is complementary to an mRNA codon.
Anticodon: AUG
Codon: UAC
carried by a tRNA (transfer RNA).

23. Codon to Anticodon practice (mRNA  tRNA)
AUG GUA AAA UUU AAU GGC UAG
tRNA
Anti-codon
UAC CAU UUU AAA UUA CCG AUC

24. Ribosomes consist of two subunits.
The large subunit has three binding sites for tRNA.
The small subunit binds to mRNA.

26. 1. tRNA binds to a start codon (AUG) and signals the ribosome to assemble.
Amino acid
AUG
Start codon

27. 2. A complementary tRNA anticodon binds to the exposed codon

28. 3. The ribosome helps form a polypeptide bond between the amino acids.
The ribosome pulls the mRNA strand the length of one codon.

29. 4. The now empty tRNA molecule exits the ribosome.
A complementary tRNA molecule binds to the next exposed codon.

30. 5. This continues until a stop codon is reached (UAG, UAA, or UGA)
-The ribosome releases the completed polypeptide chain, now a protein, and disassembles.
Finished PROTEIN

31. Protein Synthesis in a Nutshell
Replication
DNA A A G A C G T A G
DNA ______ ______ _______
(transcribe this piece)
Transcription
mRNA ______ ______ _______
Translation
tRNA ______ ______ _______
codons
___
___
___
Amino Acids
anticodon
Finished Product:____________!!!!!!

32. Protein Synthesis in a Nutshell
Replication
DNA A A G A C G T A G
DNA ______ ______ _______
(transcribe this piece)
Transcription
mRNA ______ ______ _______
Translation
tRNA ______ ______ _______
T T C T G C A T C
A A G A C G U A G
codons
Lys Thr Stop
Amino Acids
U U C U G C A U C
anticodon
Finished Product:____________!!!!!!
A Protein

33. Transcription

34. Translation

35. Protein Synthesis; Translation Video 2m

36. Goal: At least 5 proteins synthesized Each extra= EXTRA CREDIT
Protein Synthesis Lab
In Pairs…
1. Get a DNA “Code #” from Mrs. M
2. Go to the Nucleus
Write down the DNA strand
Transcribe DNA into an mRNA strand
3. Go to the Ribosome (Rough E.R.)
Write the tRNA anticodons
Flip over anticodon cards to reveal secret message
4. Show Mrs. M
Get a new code
Goal: At least 5 proteins synthesized
Each extra= EXTRA CREDIT

37. Explain the connection between a codon and an amino acid.
Compare the process of translation to translating Spanish into English.
Compare Start and Stop codons to the beginning and end of a sentence.
What is an anticodon?
For # use the amino acid chart on pg. 244 to find which amino acids would be encoded by the mRNA codes below:
GCC
AAA
AGC
UAG
AUA
AAU
GCA
CCG
UCA
AGG

38. Explain the connection between a codon and an amino acid.
A codon is a three-nucleotide sequence that codes for an amino acid.
Compare the process of translation to translating Spanish into English.
Just as we could translate English into Spanish, cells can translate an RNA message into amino acids, to build proteins.
Compare Start and Stop codons to the beginning and end of a sentence.
A start codon (like the first word in a sentence) codes for the start of translation and the start of an amino acid chain, the stop codon codes for the end of the amino acid chain (like the period at the end of a sentence).
What is an anticodon?
A set of three nucleotides that is complementary to an mRNA codon.
GCC- alanine
AAA- Lysine
AGC- Serine
UAG- stop codon
AUA- Isoleucine
AAU- Asparagine
GCA- Alanine
CCG- Proline
UCA- Serine
AGG- Arginine