1. Microtubule motors Text and image sources are included using the notes function of this file

2. Intracellular transport vesicles VSVG-GFP and Microtubules

3. Fast Axonal Transport in vivoin vitro Loligo

4. Freeze! 50 nm microtubule kinesin Transport vesicle

5. Biophysical analysis 8nm steps correspond to the spacing of tubulin dimers Kinesin develops about 6 picoNewtons (pN) of force

6. Conventional Kinesin Two heads are better than one! ATP

7. Kinesin model

8. In vitro motility Fluorescent microtubules gliding over a kinesin-coated surface Kinesin

9. Biotech saddles the beast A “rectifying” shape can direct traffic

10. Kinesins share a conserved motor domain, placed in different evolutionary contexts 100 nm

11. Turning tail Ionic strength (in vitro) Post-translational modification? Folding modulates interactions with microtubules and cargo 400kDa Conventional Kinesin

12. Kinesin in vitro motility assays Using polarity marked microtubules Conventional kinesin is a plus-end directed motor (minus-end of track leads) Ncd is a kinesin that side steps in a weakly minus- end directed motion to rotate the track - + seed

13. Stepping out

14. Kinesin family tree

15. MAPs can block motor activity Kinesin-driven anterograde movement of mitochondria is blocked by ectopic expression of MAPs like Tau

16. Cytoplasmic dynein

17. Adapting to a membrane

18. Cytoplasmic dynein and  -COP

19. Dynein inhibition causes outward dispersal of lysosomes

20. Dynein family tree Spotty, perinuclear localization Golgi localization Flagellar dyneins

21. Chlamydomonas

22. Flagellar structure

23. The flagellar dynein motor is (a) complex

24. Microtubule sliding powers flagellar beating

25. Sliding disintegration

26. Dynein Mechanochemical Cycle

27. Axonemal abnormalities Normal Kartagener’s syndrome

28. Keeping the beat!

29. Regulation

30. Building the flagellum

31. Microtubule commuters kinesin dynein