1. Biomedical Imaging and Sensing Andrei Zvyagin Centre for Biophotonics and Laser Science Physics, School of Physical Sciences Biomedical Engineering, School of Information Technology and Electrical Engineering The University of Queensland

2. Projects Coherence-domain imaging techniques, as applied to cells and live biological tissue  Full-field Fourier domain optical coherence tomography (3F-OCT)  Synthetic-aperture confocal microscopy Dialysis-assisted fibre optic spectroscopy for drug sensing in situ

3. Full-field Fourier domain OCT 3D-data cube is acquired Contains comprehensive information on the sample 3D structure and its optical scatter Signal-to-noise ratio is optimized Suitable for ophthalmology applications in vivo Relies on rapid broadly-tuneable laser swept-source

4. Dialysis-assisted fibre optic spectroscopy Fluorophores Tissue constituents Illumination To spectrum analyzer Setup for measuring fluorophore concentration in tissue using DAFOS. Inset, DAFOS sampling head. Illumination light guided through a fibre F, is emerging from the dialysis membrane DM, tip. Illumination fibre Microdialysis membrane Tissue Collection fibre Needle ibreibre F DM Small molecules, e.g. drugs, enter the sampling head and detected spectroscopically Compared with traditional microdialysis tool, DAFOS is rapid The equilibrium between the solute and intercellular fluid is established via diffusion, DAFOS is potentially sensitive and reliable The use of high-fluence UV radiation is now possible, as DAFOS can be light- tight, and prevents large protein complexes from entering the sampling head Enables in vivo applications of a range of spectroscopic techniques

5. DAFOS Time response of DAFOS signal Setup for measuring fluorophore concentration in tissue using DAFOS. Inset, DAFOS sampling head. Illumination light guided through a fibre F, is emerging from the dialysis membrane DM, tip.

6. The preliminary numerical simulations considering existing tapering technology. The ultimate sub-nM sensitivity, Lize et al., Opt Expr 12 (2004) Tong et al., Nature 426 (2003) Centre for Ultra-high Bandwidth Devices for Optical Systems (CUDOS) Absorption DAFOS