1. Th1 cellsTh2 cells beta1 beta7 alpha4 IFN-  IL-4 IFN-  IL-4 Th1 cells Th2 cells Mean fluorescence Intensity (log scale) Fig. 1A Fig. 1B

2. Rolling Sticking 0 10 20 30 40 50 60 1200 2500 5000 Th2 cells VCAM-1 sites/  m 2 ) 0 10 20 30 40 50 60 Th1 cells 1200 2.500 5000 0 10 20 30 40 50 60 300 7001500 Th2 cells MAdCAM-1 (sites/  m 2 ) 0 10 20 30 40 50 60 Th1 cells 300 7001500 Number of cells/0.2 mm 2 /30 sec Fig. 1C

3. CXCR3 CCR4 Th1 cells Th2 cells I-TAC/CXCL11 0.0 200.0 400.0 600.0 800.0 1000.0 1200.0 1400.0 1600.0 1101001000 Th1 Th2 Chemokine concentration (ng/ml) MDC/CCL22 0.0 500.0 1000.0 1500.0 2000.0 2500.0 110100 1000 Chemotaxis (arbitrary units) Mean fluorescence Intensity (log scale) Th1 Th2 Fig. 2A Fig. 2B Fig. 2C

4. Intracellular Calcium Mobilization (MFI) CCL22CCL17 Time (s) 0 100 200 300 400 500 Th2 cells Th1 cells 0 100 200 300 400 500 CXCL11CXCL9 Time (s) Th1 cells 0 100 200 300 400 500 CXCL9 CXCL11 Time (s) CCL22 CCL17 Time (s) 0 200 400 600 800 1000 L1.2-hCCR4 cells CCL22CCL17 Time (s) 0 200 400 600 800 1000 L1.2-hCCR4 cells Th2 cells CCL17CCL22 Time (s) 0 100 200 300 400 500 Th2 cells Fig. 2D

5. CCR4= 8.4 beta1=144 beta7=165 CXCR4=20 alfa4=182 CXCR3=54 TH1

6. CCR4= 43.9 beta1= 270 beta7= 217 CXCR4= 33.9 alfa4= 277 CXCR3= 8 TH2

9. Adhesive substratum Chemokine Glass capillary 2 dynes/cm 2 Dynamic cellular interactions under flow conditions

10. Interactions of Th1 and Th2 cells with MadCAM-1 under flow

11. Th2 cells 0 10 20 30 40 50 Total interactions RollingSticking >10 s None MDC TARC Th1 cells 0 10 20 30 40 50 Total interactions RollingSticking >10 s MAdCAM-1 Number of cells in 0.2 mm 2 /30s None MDC TARC

12. Interactions of Th1 and Th2 cells with MadCAM-1 under flow Number of cells in 0.2 mm 2 /30s 0 20 40 60 80 100 120 None IP-10 Total interactions RollingSticking >30 s Th1 cells 0 20 40 60 80 100 120 Total interactions RollingSticking >30 s None IP-10 Th2 cells MAdCAM-1 0 20 40 60 80 100 120 None SDF-1 Total interactions RollingSticking >30 s 0 20 40 60 80 100 120 None SDF-1 Total interactions RollingSticking >30 s Th2 cells Th1 cells Number of cells in 0.2 mm 2 /30s MAdCAM-1

13. Interactions of Th1 and Th2 cells with VCAM-1 under flow

14. 0 20 40 60 80 100 120 Total interactions RollingSticking > 10s None IP-10 Th2 cells VCAM-1 Th1 cells 0 20 40 60 80 100 120 None IP-10 Total interactions RollingSticking > 10s Number of cells in 0.2 mm 2 /30s