1. Organizing the cell: the Cytoskeleton Microtubules Microfilaments Intermediate Filaments

2. MicrotubulesMicrotubules Text and image sources are included using the notes function of this file

3. EM views

4. FtsZ: a prokaryotic ur-tubulin?

5. The Tubulin Superfamily alphaPart of heterodimerAll Eukaryotes betaPart of heterodimerAll Eukaryotes gammaPart of microtubule initiation complex All Eukaryotes deltaCentriolar component (c-tubule) Various epsilonLocalized to centriole in a cell-cycle specific manner Various etaBasal body componentParamecium zetaBasal body componentTrypanosomes FtsZComponent of filaments Prokaryotes

6. Microtubule model “Minus” end “Plus” end GTP GDP GTP GDP GTP GDP GTP Beta-tubulin “cap” exchangeable

7. Tubulin dimer atomic structure In Out Plus Minus

8. Microtubule fly-by Narrated Video

9. Microtubule initiation

10. MTOCs

11. The Centrosome revealed 9-triplet microtubule centrioles pericentriolar material singlet microtubules

12. Washout of nocodazole 10 um Successive images at 2 minute intervals after changing to drug-free media (at timepoint A)

13. Dynamic Instability in vitro Video

14. Dynamic instability in vivo Video

15. Fluorescence Speckle Microscopy Video

16. Disassembly Stathmin microtubule- binding domain In vitro assembly on axonemal microtubule seeds +/- Stathmin Activity Video

17. Katanin Video (Mitotic Extract) (Interphase Extract) Microtubule wall

18. Centrosomal release

19. Treadmilling Speckles Photobleaching

20. MAPs Note the cross- bridges (arrows) between microtubules

21. Plus end tracking proteins (+TIPS) um GFP::EB1 Cy3 Tubulin Video

22. Interrelationship between microtubules and microfilaments Microtubule effects Microfilament effects Video

23. Pathways  cof B cof E cof C CCT cof D cof A  GTP stathmin PAK Rac  GDP Dynamic Polymer Stable Polymer GEF? Rho  Tub; CLIP-170, EB-1 stabilizing MAPs; + end capture stathmin catastrophic kinesins katanin severin (EF1  ) IQGAP mDia Cdc42 Rho Synthesis and FoldingSignal transduction Assembly/Disassembly Integration with actin networks