1. Computer Aided Semen Analysis - CASA
Presented by
Vijayakumar C N
Chief Embryologist
Embryology Academy for Research and Training
26/A Raju Industrial Estate Penkar Pada Road MIRA India
Ph: / 69/ 70. Fax:
URL:

2. Introduction Inter lab and intra lab deviations in Semen Reports
Inter personal deviations in Semen Analysis Reports
Heisenberg's Uncertainty Principle

3. Requirement of CASA
To ensure indifference in semen count, motility and morphology estimates
To reduce inter personal variations and to standardize observables with higher limits of acceptance
Reliable reproducibility
Option to have databank for a patient’s records

4. Overview
High Resolution camera attached to Microscope takes video of semen sample for a prefixed time, till 200 sperms are imaged
The video is then examined through software for sperms with respect to their number, their motion characters with in the frame
User feeds the data on chamber depth, volume of sample loaded
The software now provides you with the exact count and motility as per WHO guidelines as m/ ml

5. Count and motility estimation by CASA

6. Morphometry
Stained semen smear/ unstained sample using AAV Technology (Negative phase Contrast in 40x) is exposed to the M’scope and image is grabbed through camera
100/ 200 sperm images are grabbed
Then the images are analyzed after super imposing with standard normal/ abnormal sperm templates by the software
Results are expressed as percent normal/ Abnormal
Capable of performing WHO/ Strict Criterion evaluations

7. Morphometry

8. DNA Fragmentation in Sperms
Results as rise of ROS in the sample
> ROS gives rise to Fragmented DNA
Fragmented DNA of sperms is responsible for recurrent Miscarriages post ICSI
Sperms with Fragmented DNA appear as normal sperms even under 1000x magnification

9. How to identify DNA Fragmentation
Indirectly by ROS estimation in semen
Directly by Tunnel/ SCSA/ fluorescent assay/ Halo sperm assay
Different techniques have different cut off values
Most reliable is Halo sperm technique
CASA can help in inter person variation of results with rigid limits of assay parameter

10. DNA Fragmentation analysis by CASA

11. Rationale in selecting CASA
Many manufacturer’s are there
We need a system which can test all parameters
Disposables like chambers/ slides etc., must be available from same co.,
All hardware and software must be available from same co.,

12. Which one to offer then……
Proiser ISAS Lab CASA system
Proiser is a co., from Spain formed out of Researchers in Andrology
Proiser has developed CASA systems for various sperm samples for decades
Proiser system is complete i.e., with Microscope, Negative Phase Contrast system, Camera, heated stage, Computer and software
With Proiser You can test Count, Motility, Morphology and DNA fragmentation in one software window
Disposables are developed specially for Proiser and are freely available.

13. What is offered in Proiser ISAS system

14. Constituents of Proiser ISAS CASA
What is ISAS? – Integrated Semen Analysis System which is offered in two varieties
ISAS – Complete System (user defining is Required)
ISASLAB – Complete System (ready to use)

15. ISAS – Complete System modules
ISAS-MC - motility & concentration only
ISAS-MCMf - Motility, Concentration, Morphometry/ morphology
ISAS-MCMfd - Motility, Concetration, Morphometry and DNS fragmentation

16. ISASLAB – system Modules
LAB-MC – Motility & Concentration only
LAB-MCM – Motility, Concentration and Morphology (without staining, simultaneously)
LAB-MCM-STM – Motility, Concentration and Morphology with stained slides
No DNA fragmentation module in ISASLAB.

17. Thank You…