1. Regulation of Tracheal Fusion by Arf like GTPase Confusion DT DB LT GB

2. Arf like GTPase Arf family GTPase ( ADP-ribosylation factor GTP binding proteins): Arf, Arf like and Sar Arf like GTPase  Arl1, ARFRP : TGN (trans-Golgi network)  Arl2, Arl3: Microtubule biogenesis Cfu is Arl3 homologue

3. Confusion mRNA in Fusion Cells cfu

4. Confusion Expression in Tracheal Fusion Cells Merge Cfu Dys

5. Cfu Mutants Show Tracheal Fusion Defects w1118d10234/d10234 Cfu RNAi control

6. Cfu Mutants d10234 w1118 d10234/d10234

7. Fusion Cell Identity D10234/TM3 twi-GFPD10234/D10234 DSRF Dys

8. Fusion Process E-Cadherin Cytoskeletal track: shortstop, F-actin, microtubule Peter A. Kolodziej et. al Development, 2003

9. Cfu localization in Fusion Cells Actin Tublin Cfu Merge

10. Sequence Analysis of Cfu G2AT31N: negativeQ72L: active

11. Cfu Q72L Transfected S2 Cell Merge Cfu ActinTublinDapi Cfu Q72l S2

12. Potential Role of Cfu in Trafficing No Colchicine

13. Potential Role of Cfu in Trafficing Colchicine: prevent microtubule polymerization

14. Latrunculin: prevent actin polymerization Potential Role of Cfu in Trafficing No Latrunculin

15. Potential Role of Cfu in Trafficing Latrunculin: prevent actin polymerization

16. Future Directions UAS-cfu, UAS-cfuQ72L, UAS-cfu T31N rescue cfu mutants Endsout to generate cfu mutants Time-lapse movie: UAS-actin-GFP / btl, gal4; d10234/d10234 »UAS-tubulin-GFP / btl, gal4; d10234/d10234 UAS-shot-GFP / btl, gal4; d10234/d10234 RNAi screening to identify genes interact with Cfu

17. Endsout * * I-SceI site FRT Flip, I-SceI Target gene wi *