1. Quad-Rat Vitals Monitor Advisor: Paul Thompson Client: Dr. Alex Converse

2. Overview PET Imaging Requirements Existing Devices Previous Semester’s Work Pulse Oximeter Probe Design Options LabVIEW Interface Design Options Where do we go from here? Acknowledgments

3. PET Imaging of Rats Positron emission tomography (PET) scans to monitor location of tracers in rats Client scans four anesthetized rats at a time ◦ Needs to monitor the rats’ vitals http://homepages.cae.wisc.edu/~bme300/rat_monitor _s09/reports/FinalPaperSpring09.pdf

4. Requirements: Quads Vitals Monitor Client needs to simultaneously monitor four rats 1)SpO 2 levels, accuracy of ±2% 2)Heart rates up to 500 beats per minute 3)Respiratory rates of 20 to 30 breaths per minute 4)Rectal temperatures of 93 - 100° F Non-invasive probes and user-friendly interface

5. Existing Devices Existing devices: ◦ Client currently uses rudimentary devices to monitor vitals ◦ Available: Pulse oximeter for mice and rats  Over $7000, one rat at a time http://www.starrlifesciences.com/mri.html

6. Previous Semester’s Work Developed circuit for detecting breathing rate using force sensing resistor Designed and calibrated thermistor to detect temperature Designed Graphical User Interface (GUI) in LabVIEW displaying breathing rate and temperature Attempted to reverse engineer existing Pulse Oximeter Force sensing resistor LabVIEW interface

7. General Probe Requirements Applicable for rats of varying size Reusable Sufficient blocking of ambient light Compatible LED and Photodiode http://www.oximetry.org/pulseox/principles.htm

8. Foot Clip Pros: ◦ Similar to current device ◦ Easy to reposition ◦ Positioned far from the head Cons: ◦ Can displace easily ◦ Risk of LED/Photodiode misalignment ◦ Potential to apply excessive pressure

9. Foot Wrap Pros: ◦ No displacement once attached ◦ Alignment of LED/Photodiode maintained throughout testing Cons: ◦ Not adjustable ◦ More involved cleaning process

10. Tail Clip Pros: ◦ Easy to attach  No confusion over placement location ◦ Adjustable Cons: ◦ May interfere with catheter in tail ◦ Potential to apply excessive pressure

11. Design Matrix – Pulse Oximeter Probe Criteria (Weight) Foot ClipFoot WrapTail Clip Ergonomics(20)181214 Cost (5)454 Accuracy (50)46 35 Safety (10)898 Durability (10)969 Feasibility (5)454 Total (100)898374

12. Four Corners with Buttons Full display

13. Four Corners with Buttons Pros: ◦ Logical layout ◦ Important information visible Cons: ◦ Extra clicking ◦ History graphs incomparable ◦ Less robust A sample quarter of the final display

14. Tab Interface Pros: ◦ Uncluttered interface ◦ Easy to program Cons: ◦ Inefficient ◦ Data hard to compare Full display

15. Four Corners with Histories Full display

16. Four Corners with Histories Pros: ◦ All data on one screen ◦ Easy to compare trends Cons: ◦ Cluttered interface ◦ Tiny on small screen A sample quarter of the final display

17. Design Matrix – LabVIEW Interface Criteria (Weight) 4 Corners with Buttons Tabs4 Corners with Histories Ergonomics (45) 382544 Programming Feasibility (35) 263230 Aesthetics (20)151814 Total (100)797588

18. Where do we go from here? Finish LabVIEW GUI ◦ Determine how data will be collected from Pulse Oximeter Design pulse-ox probe to fit rat’s hind paw ◦ Integrate with signal received from Graduate Students’ circuit Print circuit boards Fabricate housing apparatus

19. Acknowledgements Professor Paul Thompson Dr. Alex Converse Amit Nimunkar Tim Balgemann Liz Ahlers Nate Werbeckes

20. Questions?