1. Extraction of Nicotine By: DJ Hall and Brittany Hicks

2. Purpose Determine if nicotine is extractable by means of supercritical fluid Run more than one sample type to determine if this makes a significant difference

3. SFE Supercritical Fluid Extraction Uses high pressure CO 2 Used to extract organics from different types of matrixes Allows for easy separation Problems All soluble organics will be separated form matrix

4. GC-MS Gas Chromatography Mass Spectroscopy Separation of violate organics Determine the analyte separated at specific retention times using MS

5. Nicotine (162.12g/mol) Poison (parasympathomimetic alkaloid) Formed in nightshade family of plants Antiherbivor chemical Small doses Stimulant Relaxation Large doses Fatal

6. Extraction 2 Cigarettes Marlboro blend 27 2 Pieces Nicorette Gum 2mg 50 Drops of e-cigarette liquid 2.4% by weight

7. Extraction cont. All extractions were obtained by bubbling into methanol and using SFE methanol modifier pump Cigarettes Ground using mortar and pestle Nicorette Gum Cut into small pieces E-cigarette liquid Celite as inert matrix

8. Determination of Nicotine All samples were diluted to 50ml of methanol A standard was created by diluting 4mg of Nicotine in 50ml of methanol Standards and Samples were run using the GC-MS

9. Reference Mass Spectra

10. Standard Nicotine peak

11. Cigarettes Nicotine peak

12. Cigarettes cont.

13. E-Cigarette Liquid Nicotine peak

14. E-Cigarette Liquid Cont.

15. Nicorette Gum

16. Chromatogram Comparison E-Cigarette Liquid Nicorette Gum Cigarette Standard

17. Conclusion It is possible to extract nicotine using the SFE Both the E-Cigarette and Cigarette samples contained nicotine The Nicorette Gum showed undetectable amounts of nicotine Nicotine may have be contained in the gum residue that was filtered before dilution

18. Future Project Quantify the amounts of nicotine extracted Determine the extraction efficiency

19. Work Cited Fischer, M. and Jefferies, T. Optimization of Nicotine Extraction from Tobacco Using Supercritical Fluid Technology with Dynamic Extraction Modeling. J.Agric. Food Chem. (1996), 44, 1258-1264.