1. ORGANELLES OF THE CYTOPLASM

2. Cytoplasm Located between plasma membrane and nucleus Cytosol Water with solutes (protein, salts, sugars, etc.) Cytoplasmic organelles Metabolic machinery of cell

3. Cytoplasmic Organelles Membranous Mitochondria Peroxisomes Lysosomes Endoplasmic reticulum Golgi apparatus Nonmembranous Cytoskeleton Centrioles Ribosomes

4. Mitochondria Double-membrane structure with shelflike cristae Provide most of cell’s ATP via aerobic cellular respiration Contain their own DNA and RNA

5. Figure 3.17 Enzymes Matrix Cristae Mitochondrial DNA Ribosome Outer mitochondrial membrane Inner mitochondrial membrane (b) (a) (c)

6. Ribosomes Site of protein synthesis Free ribosomes synthesize soluble proteins Membrane-bound ribosomes synthesize proteins destined for membranes or secretion

7. Endoplasmic Reticulum (ER) System of Interconnected tubes and parallel membranes enclosing cisternae Continuous with nuclear membrane Two varieties: Rough ER Smooth ER

8. Figure 3.18a Nuclear envelope Ribosomes Rough ER Smooth ER (a) Diagrammatic view of smooth and rough ER

9. Rough ER External surface studded with ribosomes Manufactures all secreted proteins Synthesizes membrane integral proteins and phospholipids

10. Smooth ER Its enzymes catalyze reactions involved with: In the liver-Lipid metabolism, cholesterol synthesis, synthesis of lipid components of lipoproteins In testes: Synthesis of steroid-based hormones (testes) In skeletal & cardiac muscle—storage & release of calcium

11. Golgi Apparatus Stacked and flattened membranous sacs Modifies, concentrates, and packages proteins and lipids Proteins exit via secretory vesicles

12. Figure 3.20 Protein- containing vesicles pinch off rough ER and migrate to fuse with membranes of Golgi apparatus. Proteins are modified within the Golgi compartments. Proteins are then packaged within different vesicle types, depending on their ultimate destination. Plasma mem- brane Secretion by exocytosis Vesicle becomes lysosome Golgi apparatus Rough ER ER membrane Phagosome Proteins in cisterna Pathway B: Vesicle membrane to be incorporated into plasma membrane Pathway A: Vesicle contents destined for exocytosis Extracellular fluid Secretory vesicle Pathway C: Lysosome containing acid hydrolase enzymes 1 3 2

13. Lysosomes Spherical membranous organelles containing digestive enzymes (acid hydrolases) Can degrade/breakdown: Ingested bacteria, viruses, and toxins Nonfunctional organelles Break down and release glycogen Break down bone to release Ca2 +

14. Figure 3.22 Golgi apparatus Transport vesicle Plasma membrane Vesicle Smooth ER Rough ER Nuclear envelope Lysosome Nucleus

15. Peroxisomes Membranous sacs containing powerful oxidases and catalases Detoxify harmful or toxic substances Neutralize dangerous free radicals

16. Cytoskeleton Elaborate series of rods throughout cytosol Microtubules -hollow tubes, mostly radiating from a centrosome; determine overall shape of cell and distribution of organelles Microfilaments -actin strands attached to cytoplasmic side of plasma membrane; Involved in cell motility, change in shape, endocytosis and exocytosis Intermediate filaments-Tough, insoluble ropelike protein fibers ;Resist pulling forces on the cell and attach to desmosomes

17. Figure 3.23a Strands made of spherical protein subunits called actins (a) Microfilaments Actin subunit Microfilaments form the blue network surrounding the pink nucleus in this photo.

18. Figure 3.23b (b) Intermediate filaments Tough, insoluble protein fibers constructed like woven ropes Fibrous subunits Intermediate filaments form the purple batlike network in this photo.

19. Figure 3.23c (c) Microtubules Hollow tubes of spherical protein subunits called tubulins Tubulin subunits Microtubules appear as gold networks surrounding the cells’ pink nuclei in this photo.

20. Centrioles Involved in: cell division Found within the centrosome

21. Figure 3.25a Centrosome matrix (a) Centrioles Microtubules

22. Cellular Extensions Flagella Whiplike, motile extensions on surfaces of certain cells propel whole cells (tail of sperm) Cilia move substances across cell surfaces

23. Figure 3.26 Plasma membrane Outer microtubule doublet Dynein arms Central microtubule Radial spoke TEM Triplet Basal body (centriole) Cilium Microtubules Plasma membrane Basal body Cross-linking proteins inside outer doublets Cross-linking proteins inside outer doublets A longitudinal section of a cilium shows microtubules running the length of the structure. The doublets also have attached motor proteins, the dynein arms. The outer microtubule doublets and the two central microtubules are held together by cross-linking proteins and radial spokes. A cross section through the basal body. The nine outer doublets of a cilium extend into a basal body where each doublet joins another microtubule to form a ring of nine triplets. A cross section through the cilium shows the “9 + 2” arrangement of microtubules. TEM

24. Figure 3.27 (a) Phases of ciliary motion. (b) Traveling wave created by the activity of many cilia acting together propels mucus across cell surfaces. Power, or propulsive, stroke Layer of mucus Cell surface Recovery stroke, when cilium is returning to its initial position 1234567

25. Cellular Extensions Microvilli Fingerlike extensions of plasma membrane Increase surface area for absorption

26. Figure 3.28 Microvillus Actin filaments Terminal web

27. Nucleus Genetic library with blueprints for nearly all cellular proteins Responds to signals and dictates kinds and amounts of proteins to be synthesized Surrounded by nuclear envelope (perforated for exit of material) Contains threadlike strands of DNA (30%), histone proteins (60%), and RNA (10%) called chromatin Also contains nucleoli : dark-staining spherical bodies involved in ribosome assembly

28. Figure 3.29a Chromatin (condensed) Nuclear envelope Nucleus Nuclear pores Nucleolus Cisternae of rough ER (a)

29. Figure 3.29b Nucleus Nuclear pores Fracture line of outer membrane Nuclear pore complexes. Each pore is ringed by protein particles. Surface of nuclear envelope. Nuclear lamina. The netlike lamina composed of inter- mediate filaments formed by lamins lines the inner surface of the nuclear envelope. (b)

30. Figure 3.30 Metaphase chromosome (at midpoint of cell division) Nucleosome (10-nm diameter; eight histone proteins wrapped by two winds of the DNA double helix) Linker DNA Histones (a) (b) 1 DNA double helix (2-nm diameter) 2 Chromatin (“beads on a string”) structure with nucleosomes 3 Tight helical fiber (30-nm diameter) 5 Chromatid (700-nm diameter) 4 Looped domain structure (300-nm diameter)