1. Cell Transport BLOCK Day: notes & Lab set up

2. Bellwork
What is concentration? (NOT the kind where you’re thinking really hard…)
Why is the orange juice you mix with water called “concentrate”?
Draw a picture showing the difference between the molecules in a LOW concentration solution vs a HIGH concentration solution
What is the difference between being passive and being active?

3. Cell Membrane Notes (in notebook)
Copy down only most important things from each slide.
ON ODD PAGES
INFO
3 drawings/diagrams
1 memory strategy
3 ?
summary

4. On Slates: name that membrane part (1-5)2
On Slates: name that membrane part (1-5)
phospholipid 5
Which side is the inside of the cell and which is the outside? 3 1 4

5. MYON – Diffusion & Osmosis
Semi-permeable
Solute
Solvent
Solution
Simple Diffusion
Concentration Gradient
Osmosis
Golden Rule of Osmosis
Hypotonic
Isotonic
Hypertonic

6. Being Semi-permeable Selective Permeability
Certain molecules can pass through and others are prevented.
Which part of the cell is semi-permeable?
Look around the room, can you name some things that are permeable and not permeable?

7. Why is permeability important?
Small molecules, such as O2 & CO2, can enter and leave the cell freely.
Large molecules such as proteins and carbs can’t.
Several processes are involved in moving materials across the membrane.
THINK: Which is passive and which is ‘active’?

8. What do these key terms mean?
SOLUTION
Solute
Solvent
What are examples of each?
Solution
Solute
Solvent

9. Concentration
Concentration: How close together molecules are in a solution; the amount of solute in a particular amount of solvent
Which beaker has a higher concentration of food coloring?

10. Passive Transport
A concentration gradient is a measurement of how the concentration of something changes from one place to another.

11. Passive Transport No energy required
Solutes are moved down the concentration gradient:
Osmosis
Diffusion
Facilitated diffusion

12. Passive Transport
Osmosis- the movement of water across a semi-permeable membrane from an area of low solute concentration to an area of high solute concentration

15. Hear Ye! Hear Ye! This is the Golden Rule of Osmosis:
The water follows the solute!

16. The water follows the solute!
Why? You ask…
If the solute could spread out, it would. ( The Laws of Physics DEMAND it )
But when a semi-permeable membrane stops them, there is only one thing that can move
What is that?

17. The Solvent
Which in the case of almost every single biological situation is going to be our good friend…
WATER

18. The WATER will move to make the spaces between the solute even out
The WATER will move to make the spaces between the solute even out. This might mean the cell shrinks or swells up. The water doesn’t care what the effect is on the cell because in OSMOSIS…

19. The water follows the solute!

22. BELLWORK: Explain & diagram what is happening when you drop a plant cell into distilled water, and into salt solution. Label (solute and solvent) and water movement using an arrow.

23. Passive Transport
Simple Diffusion- movement of molecules (a solute) from an area of high concentration to an area of low concentration

24. Simple Diffusion- movement of molecules (a solute) from an area of high concentration to an area of low concentration

25. But wait, what’s a solute?+ =
Solute
(Kool-Aid powder)
Solvent
(water)
Solution
(Kool-Aid)
Solute: a substance that is dissolved in something else (the solvent).

26. Small dish of water Simple Diffusion “Molecules” of Kool-Aid
What will happen to the “molecules” of Kool-Aid?
They will diffuse!
“Molecules” of Kool-Aid

27. Slate Work
Draw where the solute (Kool-Aid molecules) will be in 10 hours.
Write out the word for this process.
Think to yourself: did this happen all at once? What was the process?

28. Concentration Gradient:
Simple Diffusion
Concentration Gradient:
difference in concentration of a solute across a region
How would you describe this concentration gradient?

29. Putting it all together so far…
Solutes diffuse along a concentration gradient from an area of high concentration to an area of low concentration.
Which way will the solute diffuse?

30. What does that say about diffusion and the use of energy?
For this to happen is stirring required?
Diffusion…it just happens.
Too much stirring.

31. With your partner… Partner #1 – explain Osmosis and how is works!
Partner #2 – explain simple diffusion and how it works!

32. Dialysis Tubing Is also IMPERMEABLE to sucrose…
Which is good, because I made a bunch of sucrose solutions of different concentrations, and I can’t remember which is which.

33. Dialysis Lab – Your Job
Objective: To determine which of the unknown solutions is High, Medium and Low concentration.
Purpose: To observe the process of osmosis first hand and to use this process to understand how dialysis works.

34. Materials
3 Unknown colored solutions (High, Med and Low Concentration)
Water (150 mL)
3 beakers*
3 pieces of dialysis tubing*
3 pipettes
Scissors
Scale
Paper towel
PROCEDURES: Watch demo, and write down procedures

35. Lab Title: Osmosis Lab (+ catchy title)
Problem/Question: Does ______________ of _________ in the bag affect the final ________ of the bag in ___ after in soaks in water.
Hypoth:
if _________________ (color solution) has (highest/lowest solute concentration) relative to the
Then _________________
As measured by __________

36. Data table: Mass of dialysis tubes in grams
COLOR
INITIAL
FINAL
Difference
High/Low/Med
Red
Blue
Yellow
Diagram:
Draw all 3 set ups at the start of the experiment and 15 minutes later.
-Include particulate drawings showing H20, and sucrose molecules.
-Label the solute and the solvent.
-Use arrows to show the flow of water

37. Materials:
Procedures: 
Data Table:
Qual. Observations
Graph: line/bar
Conclusion