1. INDIA /BRAZIL / SOUTH AFRICA TRI-LATERAL
COLLABORATIVE PROGRAM ON SCIENCE AND TECHNOLOGY
II Forum IBSA on Malaria, Teresina – PI, BR, March, 7th - 8th, 2006
Hernando A del Portillo, PhD Departamento de Parasitologia
Instituto de Ciências Biomédicas Universidade de São Paulo

2. Diagnosis, Cell and Molecular Biology

3. DIAGNOSIS

5. Giemsa-staining Malaria Diagnosis Brazil 1960-2005

6. External Evaluation of Giemsa-staining diagnosis in reference laboratories from the State of Pará Dra. Marinete Póvoa
State of Pará more than cases/2005
11 participating laboratories
Five different reference slides: P. falciparum, P. vivax, mixed infection, P. vivax + T. cruzi, negative control
73% of the labs diagnosed correctly all slides, remaining 27% miss-diagnosed 1 slide.
None of the labs identified T. cruzi.
5 labs have adequate training; 4 labs require re-training and 2 labs require capacitance.

7. Multi-Center Assesstment of Immuno-chromatographic Methods
Coordinated: Prof. Cor Jésus Fernandes Fontes

8. Giemsa Staining Total No of Patients : 539 Resuts given by
Manaus, Porto Velho, Macapá e Cuiabá.
Giemsa Staining F V N
Total 87 2 89
OPTIMAL V*
104 4 9
333
346 T
otal 91
113
335
539 * =
Positive results of other humana malaria parasite species,
here in considered positive for
P. vivax
Sensitivity
P.falciparum
= 95,6%
P.vivax
= 92,0
Specificity
= 99,5%
= 100,0%
Overall sensitivity for malaria diagnosis
= 93,6%
Overall specificity for malaria diagnosis
= 99,4%

9. PCR

11. NESTED-PCR

12. NESTED-PCR
Evaluation of negativation and detection of three human malaria parasite species: Marinete Póvoa
Blood bank surveys Ricardo Machado
MF Ferreira-Da-Cruz
Mixed Infections Graça Alecrim
Asymptomatics LH Pereira da Silva

13. Nested-PCR and Malaria Diagnosis at FMT/AM
Nested-PCR detected 14.5% of mixed infections as opossed to 1,8% detected by Giemsa staining

14. Asymptomatic patients

16. ELISA
Determination of the cutoff value for IgG-ELISA-P. vivax MSP1-19, using the Receiver Operating Characteristic (ROC) Curve.
Sandra do Lago Moraes-Avila & Antonio Walter Ferreira

18. Nogueira P, Alves F et al

19. Giemsa-staining Malaria Diagnosis Brazil 1960-2005

20. MOLECULAR AND CELL BIOLOGY

22. Polymorphims of Molecular Markers - Parasites
P. vivax Duffy Binding Protein (de Brito, Moreira, Carvalho)
P. falciparum reticulocyte binding-like homologues (PfRh1-4) (Mariano Zalis)
P126 protein (Ferreira-da-Cruz, Banic, Carvalho, Ribeiro)
Glycophorin B (GBP), HFE (Ricardo Machado)
MSP1, MSP2 (Marcelo Urbano)

23. Baum et al PLoS Path 2005

24. Zalis, M (unpublished)

26. Microssatelites Plasmodium vivax
Cor Jesús Fernandes Fontes, Alvaro Couto, Luzia H Carvalho Cristiana FA Brito
2006

27. Polymorphisms - Human Molecular Markers and Associations with Malaria Infection
CR-1, MBL, TLR, NOS2, TNF-a, IFN-b, IL-4, IL-10
CD36, ICAM-1, Cytochrome P450, GST
(Sandra do Lago Moraes)

29. CSP
MSP119

30. Mosquitoes and cDNA libraries
cDNA library of A. darlingi
W. P. Tadei