1. Lentikats Biotechnology in Food Industry

2. Content  Our aim  What is Lentikats Biocatalyst  Production of Lentikats Biocatalyst  Benefits of Lentikats Biotechnology  Lentikats Biocatalyst in Food Industry  Our guarantees  Our partners  Contacts

3. LentiKat’s a.s. in Food Industry  LentiKat’s a.s. follows up on nine year of research and development of Lentikats Biotechnology.  Our production know-how for commercial use of our Biocatalyst and for its industrial applications is patented internationally.  Our main focus is in intensification of production processes that leads to: higher process yields enhanced process stability, Increased revenue, Reduction of environmental footprint. Biotechnologies are changing the world…

4. Lentikats Biotechnology Unique shape of the matrix and original immobilisation procedure enables to encapsulate free enzymes or microorganisms (bacteria, yeasts, fungi) inside a hydrogel porous carrier from polyvinyl alcohol (PVA). The advantages of PVA matrix are: excellent physical and mechanical characteristics that result in a long-term mechanical stability hardly biodegradable no toxic effects Lentikats Biocatalysts enable easy separation of enzymes or microorganisms from the bulk media and therefore can be used repeatedly. With biotechnologies to a better future…

5. Microencapsulation Lentikats Biotechnology spherical shape – limited diffusion to the “core” traditional proven technologynew method based on well-proven principle lentil-like shape – no diffusion limitations „micro“ size„mili“ size – easier separation vs.

6. How does the Lentikats Biocatalyst work? A maximum surface in combination with the minimum volume of Lentikats Biocatalyst results in a substantial reduction of diffusion limitations associated with the transfer of substrate into the matrix and the product out of the matrix.

7. Why polyvinylalcohol? It is an inexpensive material Encapsulation takes place under optimal, cell-sparing conditions It allows a production of hydrogel with excellent mechanical properties It does not have any side effects on fermentation It offers high capacity for the cell loading The process is stable and easy to control It is a non-toxic material Biotechnologies are changing the world…

8. How is Lentikats Biocatalyst produced?

9. Lentikats Biocatalysts Biocatalyst Types for Use in Lentikats Encapsulation: Free Cells dead cells viable, non growing cells viable and growing cells Free or Immobilised Enzymes. Lentikats Biotechnology allows to immobilise any type of enzyme The size of Lentikats Biocatalyst pores varies depending on the size and diameter of the Biocatalyst due to: PVA additive type (size of macromolecule) additive concentration biomass concentration

10. Lentikats Biocatalysts Manufacturing Plant

11. Laboratory Units for Encapsulation For low-capacity production of encapsulates (especially for research purposes), a laboratory device is available

12. What are the benefits? Higher reaction rates Reduction of reaction tanks’ volume Operational costs savings (electricity, heat) Lower production of waste and by-products Environmentally friendly disposal Matrix containing high concentration of biomass Higher yields (in comparison to conventional technologies using free biomass) Possibility of repeated usage Operational costs savings Enhanced resistance of modified and immobilised biomass Higher process stability Easy separation of biomass from reaction media Reduced costs for downstream processes (separation)

13. ..and also… Shortening of the reaction times Much higher yields in comparison to conventional technologies (up to 5 – 15 % higher) Repetitive use of the Biocatalyst, easy separation from media (sieve separator) Higher productivity of the Biocatalyst Lower risk of contamination during the production process High stability and easy control of production process Biotechnologies are changing the world…

14. Technological set-up  - inlet of reaction media  - filling of Lentikats Biocatalyst  - medium with Lentikats Biocatalyst  - stirrer  - sieve separator  - outlet of separated media  - outlet of Lentikats Biocatalyst     

15. Lentikats and food industry We currently offer solutions for the following processes:  production of lactose free milk Lentikats Biocatalyst with β -galaktosidase activity  production of invert sugar Lentikats Biocatalyst with invertase activity  whey processing Lentikats Biocatalyst with β -galaktosidase activity for: production of D-galaktose from whey lactose (pharmaceutical quality) production of galacto-oligosaccharides from whey lactose  production of special syrups Lentikats Biocatalyst with invertase activity for production of glucose-fructose syrups Lentikats Biocatalyst with immobilised glucoamylase for production of glucose from sugar and starch-based materials or glucose syrups from maltotriose or maltose syrup

16. Lentikats and food industry Following solutions are currently under development and testing :  production of organic acids Lentikats Biocatalyst with immobilised bacteria Bacillus coagulans for production of lactic acid from lactose, glucose and sucrose Lentikats Biocatalyst with immobilised filamentous fungi Aspergillus niger for production of gluconic acid from sucrose and glucose Lentikats Biocatalyst with immobilised filamentous fungi Aspergillus terreus for production of itaconic acid from sucrose and glucose Biotechnologies are changing the world…

17. Lentikats Biotechnology - examples LENTIKATS BIOTECHNOLOGY FOR PRODUCTION OF INVERT SUGAR:  enzyme: immobilised saccharase, 3-D-fruktofuranosidase for hydrolysis of sucrose into glucose and fructose.  optimum temperature 30–35°C  optimum pH 4,5–5,0  conversion rate 100 %  operational stability: 600-1000 repeated batch conversions  application - processing of molasses or sucrose solutions sourceactivity Baker’s yeast600-1000 g sucrose/ kg LB/hr

18. LENTIKATS BIOTECHNOLOGY FOR PRODUCTION OF LACTOSE FREE MILK  enzyme: immobilised laktase, β -D-galactosid galactohydrolase for lactose hydrolysis.  optimum temperature 30–50°C  optimum pH 6,0–6,5  conversion rate > 90%  operational stability: 120–160 hours Lentikats Biotechnology - examples sourceactivity Kluyveromycer lactis100-300 g GLC/ kg LB/hr

19. Investment and Investment Return Guarantees You can easily verify our great results by testing our Biocatalyst in your or other facilities with the material of your choice. Sample of Lentikats Biocatalyst free of charge Our company provides assistance and full technical support. Our company offers a long-term cooperation to companies, whose main concerns are reduced investment costs and maximal process profitability. Biotechnologies change the world…

20. Investment and Investment Return Guarantees We guarantee the following:  output parameters  operational stability of Lentikats Biocatalyst and its activity and productivity

21. Our partners in the field of industrial applications Fermenta Biotech, DIL Complex, Ghodbunder Road, Thane (West) - 400 610, Maharashtra, India www.fermentabiotech.com Protean, Pod Lesem 9, 373 16 Dobra Voda u Ceskych Budejovic, Czech Republic www.protean.cz Pharmix s.r.o., Na Sadkach 2798, 767 01 Kromeriz, Czech Republic www.pharmix.cz

22. Our partners in the field of research and development Fermenta Biotech, DIL Complex, Ghodbunder Road, Thane (West) - 400 610, Maharashtra, India www.fermentabiotech.com Institute of Microbiology, Academy of Science of the Czech Republic Vídeňská 1083, 142 20 Prague 4, Krč, Czech Republic www.biomed.cas.cz STU Bratislava, Fakulta chemické a potravinářské technologie, Ústav biotechnologie a potravinářství, Radlinského 9, 81237 Bratislava, Slovakia www.stuba.sk VŠCHT Praha, Ústav kvasné chemie a bioinženýrství, Technická 5, 166 28 Praguea 6 www.vscht.cz University of Alberta, Edmonton Alberta, T6G 2J9 Canada www.ualberta.ca Masaryk University, National Centre for Biomolecular Research, Kamenice 5, 625 00 Brno-Bohunice www.ncbr.chemi.muni.cz

23. Thank you for attention Company Headquarters: LentiKat´s a.s. Evropska 423/178 160 00 Prague 6 Czech republic Tel.:+ 420 224 362 461 Fax:+ 420 224 362 463 E-mail:info@lentikats.eu Production plant and laboratories LentiKat´s a.s. Pod Vinicí 83 471 27 Stráž pod Ralskem Czech republic Tel.:+ 420 487 851 393 Fax:+ 420 487 851 399 E-mail: info@lentikats.eu