1. Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production By Xin Li

2. To find sugar-regulating genes that direct the flow of sugar to harvested portions of the plant. Purposes

3. To find sugar-regulating genes that direct the flow of sugar to harvested portions of the plant. To produce cheaper soydiesel, more soy protein, and less expensive ethanol from the soybean plants. Purposes

4. Modeling Soybean Arabidopsis (Arabidopsis thaliana) Soybean (Glycine max)

5. Modeling Soybean Arabidopsis (Arabidopsis thaliana) Soybean (Glycine max)

6. Modeling Soybean Arabidopsis (Arabidopsis thaliana) Soybean (Glycine max)

7. Modeling Soybean Both are oilseed plants Arabidopsis has a mapped genome Insertion-induced Arabidopsis mutants are av ailable commercially

8. Modeling Soybean Both are oilseed plants Arabidopsis has a mapped genome Insertion-induced Arabidopsis mutants are av ailable commercially

9. Modeling Soybean Both are oilseed plants Arabidopsis has a mapped genome Insertion-induced Arabidopsis mutants are av ailable commercially Insertion-induced Arabidopsis mutants are available commercially

10. Insertion-Induced Mutation T-DNA Gene

11. Insertion-Induced Mutation

17. Wild-type

18. Hypersensitive mutant

19. Insensitive mutant

20. Methods Grew 300 seedlings of each of 58 different mutants and wild-type in:

21. Methods Grew 300 seedlings of each of 58 different mutants and wild-type in: 6% glucose

22. Methods Grew 300 seedlings of each of 58 different mutants and wild-type in: 6% glucose 6% sucrose

23. Methods Grew 300 seedlings of each of 58 different mutants and wild-type in: 6% glucose 6% sucrose Measured root length of seedlings grown in glucose (Gibson, 2005)

24. Methods Grew 300 seedlings of each of 58 different mutants and wild-type in: 6% glucose 6% sucrose Measured root length of seedlings grown in glucose (Gibson, 2005) Use spectrophotometry to determine anthocyanin levels of seedlings grown in sucrose (Nacry,1998)

25. Results

29. Neff and Chory (1998)

30. Results

31. Conclusion I found a mutant that is hypersensitive to 6% glucose and 6% sucrose.

32. Conclusion I found a mutant that is hypersensitive to 6% glucose and 6% sucrose. The mutant’s genotype is SALK_113292.

33. Conclusion I found a mutant that is hypersensitive to 6% glucose and 6% sucrose. The mutant’s genotype is SALK_113292. The disabled gene in mutant SALK_113292 is at1g06230.

34. Conclusion I found a mutant that is hypersensitive to 6% glucose and 6% sucrose. The mutant’s genotype is SALK_113292. The disabled gene in mutant SALK_113292 is at1g06230. The gene contains a bromodomain protein

35. Future Work Grow SALK_113292 in 1% glucose and 1% sucrose

36. Future Work Grow SALK_113292 in 1% glucose and 1% sucrose Grow SALK_113292 in 1% sorbitol and 6% sorbitol

37. Image from: htto://www.food-info.net

38. Acknowledgement Dr. Sue Gibson Dr. Chunyao Li Ms. Lois Fruen Ms. Chelen Johnson Dr. Jacob Miller Ms. Virginia Amundson Breck Advanced Team Research http://www.cbs.umn.edu/plantbio/faculty/GibsonSue/

39. Characterization of sugar-response Arabidopsis (Arabidopsis thaliana) mutants to engineer plants for higher ethanol, soydiesel and soy protein production By Xin Li