1. Two promoters of beta-glucosidase paralogues (ZmBglu2 and ZmBglu5) are highly active in tropical young maize hybrids seedlings Mario Rojas 1, Axel Tiessen 2, Felipe Ascencio 1, Carlos Angulo 1, and Gracia Gómez-Anduro 1* 1 Centro de Investigaciones Biológicas del Noroeste (CIBNOR) Av. Instituto Politécnico Nacional 195, Col. Playa Palo de Santa Rita, La Paz, B.C.S. 23096, México Email: mrojas@cibnor.mx; eangulo@cibnor.mx; ascencio@cibnor.mxmrojas@cibnor.mx eangulo@cibnor.mxascencio@cibnor.mx 2 Departamento de Ingeniería Genética, CINVESTAV, Unidad Irapuato, Km. 9.6 Libramiento Norte, 36821 Irapuato, México Email: atiessen@ira.cinvestav.mxatiessen@ira.cinvestav.mx * Corresponding author: Gracia Gómez-Anduro Email address: ggomez@cibnor.mxggomez@cibnor.mx Supplementary Material 2 Bioinformatics analysis of β-Glucosidases promoters. The sequences of the putative promoter region (2000 pb upstream start codon) of pZmBGlu2, 5, 7, 12. Showing the transcription factors binding sites. Where one is the most distant position and 2000 is the nearest to the start codon. Each analysis was performed using databases for maize, in a database reported by (Chang et al. 2008).Chang et al. 2008

2. pZmBGlu2 pZmBGlu5 pZmBGlu7 pZmBGlu12 All cis Elements

3. -300 CORE (PLACE) pZmBGlu2 pZmBGlu12

4. ABI4 (PLACE) pZmBGlu2 pZmBGlu5 pZmBGlu7 pZmBGlu12

5. ANAERO1CONSENSUS (PLACE) pZmBGlu2 pZmBGlu5 pZmBGlu7

6. ANAERO2CONSENSUS (PLACE) pZmBGlu2

7. ANAERO3CONSENSUS (PLACE)

8. pZmBGlu2 pZmBGlu5 pZmBGlu7 pZmBGlu12 C1MOTIFZMBZ2 (PLACE)

9. pZmBGlu2 pZmBGlu5 pZmBGlu7 pZmBGlu12 DOFCOREZM (PLACE)

10. DRE1COREZMRAB17 (PLACE) pZmBGlu5 pZmBGlu7 pZmBGlu12

11. DRE2COREZMRAB17 (PLACE) pZmBGlu5

12. DRECRTCOREAT (PLACE) pZmBGlu5 pZmBGlu7 pZmBGlu12

13. INTERLOWER (PLACE) pZmBGlu5 pZmBGlu7 pZmBGlu12 pZmBGlu2

14. MYBPZM (PLACE) pZmBGlu2 pZmBGlu24 pZmBGlu7

15. POLASIG3 (PLACE) pZmBGlu2 pZmBGlu5 pZmBGlu7 pZmBGlu12

16. QELEMENTZM13 (PLACE) pZmBGlu2 pZmBGlu5 pZmBGlu12

17. pZmBGlu7 TATAPVRNALEU (PLACE)

18. Dof1/MNB1A (JASPER) pZmBGlu2 pZmBGlu5 pZmBGlu7 pZmBGlu12 pZmBGlu2 pZmBGlu5 pZmBGlu7 pZmBGlu12 Dof1/MNB1A (TRANSFAC)

19. pZmBGlu2 pZmBGlu5 pZmBGlu7 pZmBGlu12 pZmBGlu2 pZmBGlu5 pZmBGlu7 pZmBGlu12 Dof2 (JASPER) Dof2 (TRANSFAC)

20. Dof3 (JASPER) Dof3 (TRANSFAC) pZmBGlu2 pZmBGlu5 pZmBGlu7 pZmBGlu12 pZmBGlu2 pZmBGlu5 pZmBGlu7 pZmBGlu12

21. PBF (JASPER) PBF (TRANSFAC) pZmBGlu2 pZmBGlu5 pZmBGlu7 pZmBGlu12 pZmBGlu2 pZmBGlu5 pZmBGlu7 pZmBGlu12

22. O2 (TRANSFAC) pZmBGlu2 pZmBGlu5 pZmBGlu7 pZmBGlu12

23. P (TRANSFAC) pZmBGlu2 pZmBGlu5 pZmBGlu7 pZmBGlu12