1. Chapter 10 Table of Contents Section 1 Discovery of DNA
DNA, RNA, and Protein Synthesis
Table of Contents
Section 1 Discovery of DNA
Section 2 DNA Structure
Section 3 DNA Replication
Section 4 Protein Synthesis

2. Section 1 Discovery of DNA
Chapter 10
Objectives
Relate how Griffith’s bacterial experiments showed that a hereditary factor was involved in transformation.
Summarize how Avery’s experiments led his group to conclude that DNA is responsible for transformation in bacteria.
Describe how Hershey and Chase’s experiment led to the conclusion that DNA, not protein, is the hereditary molecule in viruses.

3. Griffith’s Experiments
Section 1 Discovery of DNA
Chapter 10
Griffith’s Experiments
Griffith’s experiments showed that hereditary material can pass from one bacterial cell to another.
The transfer of genetic material from one cell to another cell or from one organism to another organism is called transformation.

4. Griffith’s Discovery of Transformation
Section 1 Discovery of DNA
Chapter 10
Griffith’s Discovery of Transformation

5. Chapter 10 Avery’s Experiments
Section 1 Discovery of DNA
Chapter 10
Avery’s Experiments
Avery’s work showed that DNA is the hereditary material that transfers information between bacterial cells.

6. Hershey-Chase Experiment
Section 1 Discovery of DNA
Chapter 10
Hershey-Chase Experiment
Hershey and Chase confirmed that DNA, and not protein, is the hereditary material.

7. The Hershey-Chase Experiment
Section 1 Discovery of DNA
Chapter 10
The Hershey-Chase Experiment

8. Section 2 DNA Structure
Chapter 10
Objectives
Evaluate the contributions of Franklin and Wilkins in helping Watson and Crick discover DNA’s double helix structure.
Describe the three parts of a nucleotide.
Summarize the role of covalent and hydrogen bonds in the structure of DNA.
Relate the role of the base-pairing rules to the structure of DNA.

9. Chapter 10 DNA Double Helix
Section 2 DNA Structure
Chapter 10
DNA Double Helix
Watson and Crick created a model of DNA by using Franklin’s and Wilkins’s DNA diffraction X-rays.

10. Chapter 10 DNA Double Helix
Section 2 DNA Structure
Chapter 10
DNA Double Helix
DNA is made of two nucleotide strands that wrap around each other in the shape of a double helix.

11. DNA Double Helix, continued
Section 2 DNA Structure
Chapter 10
DNA Double Helix, continued
A DNA nucleotide is made of a 5-carbon deoxyribose sugar, a phosphate group, and one of four nitrogenous bases: adenine (A), guanine (G), cytosine (C), or thymine (T).

12. DNA Nucleotides, continued
Section 2 DNA Structure
Chapter 10
DNA Nucleotides, continued
Bonds Hold DNA Together
Nucleotides along each DNA strand are linked by covalent bonds.
Complementary nitrogenous bases are bonded by hydrogen bonds.

13. Chapter 10 Complementary Bases
Section 2 DNA Structure
Chapter 10
Complementary Bases
Hydrogen bonding between the complementary base pairs, G-C and A-T, holds the two strands of a DNA molecule together.

14. Chargaff’s Rule

15. Chapter 10 Objectives Section 3 DNA Replication
Summarize the process of DNA replication.
Identify the role of enzymes in the replication of DNA.
Describe how complementary base pairing guides DNA replication.
Compare the number of replication forks in prokaryotic and eukaryotic cells during DNA replication.
Describe how errors are corrected during DNA replication.

16. How DNA Replication Occurs
Section 3 DNA Replication
Chapter 10
How DNA Replication Occurs
DNA replication is the process by which DNA is copied in a cell before a cell divides.

17. How DNA Replication Occurs, continued
Section 3 DNA Replication
Chapter 10
How DNA Replication Occurs, continued
Steps of DNA Replication
Replication begins with the separation of the DNA strands by helicases.
Then, DNA polymerases form new strands by adding complementary nucleotides to each of the original strands.

18. Section 3 DNA Replication
Chapter 10
DNA Replication

19. How DNA Replication Occurs, continued
Section 3 DNA Replication
Chapter 10
How DNA Replication Occurs, continued
Each new DNA molecule is made of one strand of nucleotides from the original DNA molecule and one new strand. This is called semi-conservative replication.

20. DNA Errors in Replication
Section 3 DNA Replication
Chapter 10
DNA Errors in Replication
Changes in DNA are called mutations.
The change in one nucleotide during replication is referred to as a point mutation.
DNA Replication and Cancer
Unrepaired mutations that affect genes that control cell division can cause diseases such as cancer.

21. Chapter 10 Objectives Section 4 Protein Synthesis
Outline the flow of genetic information in cells from DNA to protein.
Compare the structure of RNA with that of DNA.
Describe the importance of the genetic code.
Compare the role of mRNA, rRNA,and tRNA in translation.
Identify the importance of learning about the human genome.

22. Flow of Genetic Information
Section 4 Protein Synthesis
Chapter 10
Flow of Genetic Information
The flow of genetic information can be symbolized as DNA RNA protein.

23. DNA to RNA

24. RNA Structure and Function
Section 4 Protein Synthesis
Chapter 10
RNA Structure and Function
RNA has the sugar ribose instead of deoxyribose and uracil in place of thymine.
RNA is single stranded and is shorter than DNA.

25. RNA Structure and Function, continued
Section 4 Protein Synthesis
Chapter 10
RNA Structure and Function, continued
Types of RNA
Cells have three major types of RNA:
messenger RNA (mRNA)
ribosomal RNA (rRNA)
transfer RNA (tRNA)

26. RNA Structure and Function, continued
Section 4 Protein Synthesis
Chapter 10
RNA Structure and Function, continued
mRNA carries the genetic “message” from the nucleus to the cytosol.
rRNA is the major component of ribosomes.
tRNA carries specific amino acids, helping to form polypeptides.

27. Chapter 10 Transcription
Section 4 Protein Synthesis
Chapter 10
Transcription
During transcription, DNA acts as a template for directing the synthesis of RNA.

28. Section 4 Protein Synthesis
Chapter 10
Transcription

29. DNA to RNA to Protein

30. Section 4 Protein Synthesis
Chapter 10
Genetic Code
The nearly universal genetic code identifies the specific amino acids coded for by each three-nucleotide mRNA codon.

32. Chapter 10 Translation Steps of Translation
Section 4 Protein Synthesis
Chapter 10
Translation
Steps of Translation
During translation, amino acids are assembled from information encoded in mRNA.
As the mRNA codons move through the ribosome, tRNAs add specific amino acids to the growing polypeptide chain.
The process continues until a stop codon is reached and the newly made protein is released.

33. Translation: Assembling Proteins
Section 4 Protein Synthesis
Chapter 10
Translation: Assembling Proteins
Assemble a Protein

34. Chapter 10 The Human Genome
Section 4 Protein Synthesis
Chapter 10
The Human Genome
The entire gene sequence of the human genome, the complete genetic content, is now known.
To learn where and when human cells use each of the proteins coded for in the approximately 30,000 genes in the human genome will take much more analysis.