1. Unit 1 Nature of the Immune System Part 3 Acute Phase Reactants Terry Kotrla, MS, MT(ASCP)BB

2. Immunity – Very Complex System

3. Soluble Factors  Many soluble tissue and serum substances help to suppress the grow of or kill microorganisms.  Interferons - family of proteins which are important non- specific defense mechanisms against viral infections.  Transferrin - Bacteria do not thrive well in serum that contains low levels of iron but high levels of transferrin.  Complement - a group of proteins that are essential for bacterial destruction and plays an important role in both non- specific and specific immune mechanisms.

4. Acute Phase Reactants (Proteins)  Defined-normal serum constituents that increase rapidly because of infection, injury, or trauma to tissues.  Acute-phase proteins are a class of proteins whose plasma concentrations increase or decrease in response to inflammation.  This response is called the acute-phase reaction.  In response to injury local inflammatory cells (neutrophils, granulocytes and macrophages) secrete a number of cytokines into the bloodstream, most notable of which are the interleukins.  The liver responds by producing a large number of acute- phase reactants.

5. C-Reactive Protein  Increases rapidly within 4-6 hours of infection or injury.  Returns to normal rapidly once condition subsides.  Used to monitor healing and has also increased in usefulness in diagnosing Myocardial Infarction.

6. Serum Amyloid A  Major protein secreted during the acute phase of inflammation.  Has several roles, including Removes cholesterol from cholesterol-filled macrophages at site of injury – clean up. recruitment of immune cells to inflammatory sites, and Thought to play a role in cholesterol metabolism

7. Complement  A series of serum proteins involved in mediation of inflammation but also involved in opsonization, chemotaxis, and cell lysis.

8. Alpha-1 Antitrypsin  Increases during acute inflammation.  Protects tissues from enzymes of inflammatory cells, especially elastase.  When the lungs do not have enough alpha-1 antitrypsin, elastase is free to destroy lung tissue.  As a result, the lungs lose some of their ability to expand and contract (elasticity). This leads to emphysema and sometimes makes breathing difficult.

9. Haptoglobin  Binds irreversibly to free hemoglobin to protect kidneys from damage and prevent loss of iron by urinary excretion.  Haptoglobin - hemoglobin complex removed by RES, mainly spleen.  Used to monitor hemolysis

10. Fibrinogen  A coagulation factor integral to clot formation which serves as a barrier to prevent spread of microorganisms further in the body.  Levels increase with tissue inflammation or tissue destruction.  Thought to play a key role in the inflammatory response and development of rheumatoid arthritis.

11. Ceruloplasmin  Principal copper transporting protein in plasma, plays a role in iron metabolism and histamine regulation.  Stimulates the immune system to fight infections, repair injured tissues and promote healing.  Depletion found in Wilson’s disease, causes the body to absorb and retain excessive amounts of copper. Copper deposits in the liver, brain, kidneys, and the eyes. The deposits of copper cause tissue damage, necrosis (death of the tissues), and scarring, which causes decreased functioning of the organs affected. Liver failure and damage to the central nervous system (brain, spinal cord) are the most predominant, and the most dangerous, effects of the disorder.

12. References  http://www.horton.ednet.ns.ca/staff/Selig/isu/Immunity/Innate.htm http://www.horton.ednet.ns.ca/staff/Selig/isu/Immunity/Innate.htm  http://www.metacafe.com/tags/neutrophil/most_popular/ http://www.metacafe.com/tags/neutrophil/most_popular/