2. DENT 1260 Infection Control Lesson 4 Disinfection

3. Disinfection Use of liquid chemical to kill disease producing microbes. Does not kill all and does not kill spores.

4. 2. Bioburden Bioburden- the microbial material on a surface or object. This MUSt be removed in order to disinfect/ sterilize. (PRECLEAN)

5. 3. Surface vs immersion Surface- wipe/wipe or spray wipe spray technique Immersion- cover the object with the chemical

6. 4. Antiseptic, disinfectant, sterilant Antiseptic- reduces # microbes on tissue ( alcohol wipe on skin) Disinfectant- reduce # microbes on surface or object Sterilant- kills all microbes on surface / object

7. 5. Alcohol Chlorite NOT for surface or immersion Tissue antimicrobial only Sodium hypochlorite 1:10 or 1:13 Surface- harsh Immersion- OK Mix fresh Intermediate level cheap

8. 5. Iodophor Phenol Surface, immersion or tissue Intermediate level May discolor Surface Intermediate level Hard on plastics Leaves residue MCC uses Birex for surfaces

9. 5. Quats aldehydes Quaternary ammonium (with alcohol) Intermediate Surface Better on fungal See picture next slide High level Immersion only of nonporous items Sterilant, tissue preservative for biopsy

10. QUATS 1 st generation quats DID NOT HAVE alcohol and now use only to treat denture for fungus.

11. 5. Chlorhexidine gluconate Tissue antimicrobial in soap or Peridex rinse

12. 6. Microbes killed Virucidal- kills at least some virus Bactericidal- kills at least some bacteria Fungicidal- kills at least some fungi Tuberculocidal- kills TB- intermediate Sporicidal- kills spores=sterilizes Hospital disinfectant- shown to kill 3 bact- staph aureus, salmonella, and pseudomonas

13. 7. Label consideration Type of antimicrobial agent, active ingredient, microbes killed, directions of use, dilution, precautions, store and disposal info, name & address of manufacturer, volume of container Contact time EPA registration #

14. 8. Properties of “IDEAL” disinfectant Rapidly kill a broad range of microbes Residual activity Minimal toxicity Not damage surfaces Odorless and inexpensive Simple to use Good cleaner- remove bioburden

15. 9. High, medium, low level disinfectant High- kills all but not high numbers of spores (glut) Medium- intermediate- kills vegitative bact, most fungi, virus, M TB Low- is not tuberculocidal Hospital- kills staph, salmonella and Psedomonus, NOT TB

16. 9. High, Medium, low High- glutaraldehyde Medium- phenols, iodophors, quats with alcohol, sodium hypochlorite

17. 10. Surface disinfectants Phenols (synthetic) Sodium hypochlorites Iodophors Quats WITH alcohol “Bleach eQu I P ment” Use these only for surfaces in dental office!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!

18. 11. Immersion disinfection Glutaraldehyde Sodium hypochlorite Iodophor “biG” tub

19. 12. Shelf, use and reuse life Shelf life- unopened expiration date Use life- once open/mixed Resuse- life shortens the more used Ex: phenol, exp date 1yr but once mixed 21 days, bleach soln- use daily, resuse none ?

20. 13. Accidental exposure Check MSDS Wear PPE

21. The END