1. Western Blot encyclopedia

2. Western Blot Principle Western Blot Procedure
Content
Western Blot Principle
Western Blot Procedure
Western Blot Troubleshooting
Western Blot Applications
Western Blot Reagents
Signaling Pathway Images

3. Western Blot Principle
Western blot is an important technique used in cell and molecular biology. By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells.
The basic principle of western blot is to use three elements to accomplish this task:
separation by size,
transfer to a solid support, and
marking target protein using a proper primary and secondary antibody to visualize.

4. Western Blot Procedure
For more information about western blot protocol,
For detailed info about WB protocols, please visit:

5. Western Blot Troubleshooting
For more common problems in western blot and related troubleshooting, please visit:
Common Problems
Recommended Troubleshooting
Unusual or unexpected bands
Use a fresh sample which had been kept on ice
or alter the antibody;
Make sure the transfer sandwich is prepared
properly;
Change the running buffer. Learn more…
No bands
Make sure to use the right and appropriate
concentration of antibody;
Increase the conc. of antigen;
Buffers should be new and uncontaminated;
Lower the washing time. Learn more…
Faint bands or weak signal
Increase the conc. of antigen or antibody;
Increase exposure time;
Change nonfat dry milk into BSA or decrease
the amount of milk. Learn more…
High background
Lower the conc. of antibody;
Increase washing time;
Decrease exposure time. Learn more…
Patchy or uneven spots
Make sure of no air bubbles during transfer;
use a shaker for all incubation;
Change blocking buffer.

6. Western Blot Applications
Western Blot for Lyme
Western Blot for HIV
Western Blot for Herps
Western Blot for Lyme
Western Blot for FIV
Western Blot for HBV

7. Western Blot Reagents

8. Signaling Pathways – Free for download by visit