2. วัตถุประสงค์เชิง พฤติกรรม เมื่อสิ้นสุดการเรียนการสอน นักศึกษาสามารถ 1. ทราบวิธีการเก็บสิ่งส่งตรวจ ทางพยาธิวิทยาชนิดต่างๆ 2. สามารถเลือกใช้วิธีการเก็บ สิ่งส่งตรวจทางพยาธิวิทยาได้ อย่างถูกต้องเหมาะสม

3. SPECIMENS IN PATHOLOGY 1.Surgical Pathology Specimen 2.Cytopathology Specimen 3.Autopsy Specimen 4.Clinical Pathology Specimen

4. SURGICAL PATHOLOGY SPECIMEN 1.Biopsy specimen - Excisional biopsy (removal of entire lesion) (removal of entire lesion) - Incisional biopsy (part of lesion) (part of lesion)

8. SURGICAL PATHOLOGY SPECIMEN 2.Resection specimen - from surgical procedures

18. Handling of Surgical Specimen 1.Fresh tissue - avoid crushing - keep in refrigerator at 4  C - avoid wrapping with dry gauze - wrap in gauze soaked with normal saline - put in plastic bag, glass or metal containers and seal

19. Handling of Surgical Specimen 2. Fixed specimen - fixation in fixative (10% buffered formalin, alcohol, Zenker solution, B-5 solution etc.) - put the specimen in glass, plastic or metal containers with fixative and seal - put the gauze on top in case of floating specimen - volume of fixative: 10 times of the specimen - rate of fixation 1mm/hour

22. Handling of Cytological Specimen 1.Papanicolaou smear - put in 90% alcohol - spray fixation (alcohol)

23. .

26. Handling of Cytological Specimen 2. Fine Needle Aspiration (FNA) - immediately put in 90% alcohol - spray fixation (alcohol) - air dry

29. Handling of Cytological Specimen 3. Fluid (exfoliative cytology) -Fluid from cavities (pleural, pericardial, peritoneal, cerebrospinal- CSF, vitreous or aqueous humor, joint, any cavity) - Fluid from cavities (pleural, pericardial, peritoneal, cerebrospinal- CSF, vitreous or aqueous humor, joint, any cavity)

30. http://library.med.utah.edu/WebPath/INFLHTML/INFL062.html

31. http://medisuite.ir/medscape/a80640-business.html

32. http://astec.arizona.edu/education/task-trainers

33. Autopsy Specimen 1.Whole body for autopsy 2.Necropsy 3.Limited autopsy : part (s) of organ (s) or specific organ(s)

39. Clinical Pathology Specimen 1.Blood 2.Serum or plasma 3.Urine 4.Fluid from cavities (pleural, pericardial, peritoneal, cerebrospinal-CSF, vitreous or aqueous humor, joint, any cavity)

40. Laboratory Investigation in Dermatopathology 1. Special stains for skin smear - Acid fast bacilli stain (AFB) - Gram’s stain - etc.

41. Laboratory Investigation in Dermatopathology 2. KOH preparation (20%) 3. Skin smear for dark field examination for syphilis 4. Skin scraping for scabies

42. Laboratory Investigation in Dermatopathology 5. Tzanck smear for diagnosis of herpetic infection or bullous disease - for diagnosis of herpetic infection or bullous disease - new vesicle, open the roof with sharp blade - scrape at vesicle base - air dried - stain with Wright or Giemsa stains

44. Multinucleated giant cell, HSV infected

46. Viral cytopathic cells

47. Laboratory Investigation in Dermatopathology 6. Culture 7. Wood light examination - high pressure mercury arc producing UV at 360 nm (UVA)

50. Laboratory Investigation in Dermatopathology 8. Skin biopsy (excisional or incisional biopsy) - for diagnosis - for follow up - punch biopsy, shave biopsy

59. http://www.usafp.org/facultydevelopment/products/procedures/Skin/equipment.html

60. www.holemanlandscape.com