Presentation is loading. Please wait.

Presentation is loading. Please wait.

TEM GRID vs. DIME OU NanoLab/NSF NUE/Bumm & Johnson.

Similar presentations


Presentation on theme: "TEM GRID vs. DIME OU NanoLab/NSF NUE/Bumm & Johnson."— Presentation transcript:

1 TEM GRID vs. DIME OU NanoLab/NSF NUE/Bumm & Johnson

2 How do they look under microscope?
                                                                   Square Grid Hexagonal Grid 200 mesh 55% open area, 28 µm bar width Thick:17.8 µm 3 mm OU NanoLab/NSF NUE/Bumm & Johnson

3 TEM Grid Preparation There are two sides in carbon coated ultra-thin grid Formvar side - lighter side (less hydrophobic than the carbon film) Carbon film is on the darker side. OU NanoLab/NSF NUE/Bumm & Johnson

4 TEM Grid Preparation Hold the grid with tweezers.
Do not touch/contaminate the grid. Do not bend the grid. OU NanoLab/NSF NUE/Bumm & Johnson

5 TEM GRID PREPARATION Take a micropipette and put a droplet on top the grid. Let the solution sit on the grid. After min, wick away the rest of the solution with filter paper. Do not touch the grid! Finally let it sit until the grid dries off. OU NanoLab/NSF NUE/Bumm & Johnson


Download ppt "TEM GRID vs. DIME OU NanoLab/NSF NUE/Bumm & Johnson."

Similar presentations


Ads by Google