1. Reasons to Use the Dissection Video and Accompanying PowerPoint Presentation  Reduce the number of specimens used by a class  Increase the quality of the dissection for the students  Review opportunity, increasing the learning experience for the students  Student unable to dissect due to pregnancy or hypersensitivity to the preservatives  Student chooses not to dissect due to ethical/moral reasons Dissection 101: Click

2. Dissection 101: As an educator you are responsible for the implementation of the dissection activity described in the video and PowerPoint. You must have safety procedures and rules established for your classroom and make sure all of the students follow the rules to ensure a safe environment. South Dakota Public Broadcasting and Dakota State University cannot in any way be responsible or liable for any injury as a result of performing the described dissection. Complete the dissection if you feel it is appropriate and safe for your individual class. Have fun and stay safe! Click Safety Considerations  Age appropriate activity for the children in your care  Material Safety Data Sheet (MSDS) available for accident reference  Poison control number/phone readily available  Latex free gloves, eye protection and apron/lab coat  Eyewash station, shower and sink  Sharp instruments (cut away from self and others)  Sharps and specimen(s) disposal  Encourage students to report all accidents  Basic science laboratory rules (strictly enforced)

3. Click Dissection 101: Clam

4. Click Dissection 101: ClamExterior of the Clam Umbo (location): Shell originates from here (oldest section of the shell) Dorsal (hinge side) Anterior (closer to umbo) Posterior ( greater distance from umbo) Ventral (foot side - movement) Shell produced by mantel in a series of rings, similar to the rings of a tree – does not indicate age Wood inserted during preserving process to keep the clam open for dissection Orientation

5. Click Exterior of the Clam Umbo Hinge Bivalve (2 shells) Dorsal (top view)

6. Dissection 101: Clam Cut both adductor muscles on either side of the hinge; do not cut through the middle of the clam (dorsal side: posterior and anterior) Always cut away from your hands and fingers Slowly open the clam; continue to cut the adductor muscles if there is resistance. Click

7. Dissection 101: Clam Click Many times one of the mantles will rip when the clam is pulled apart. This is not part of the clam. It is an extra piece of wood accidentally left in the clam during preserving process.

8. Identify the following Structures Click Adductor muscles (2) relaxed – clam opens contracted – clam closes

9. Identify the following Structures Mantle Produces shell and provides protective and lubricated surface for the body structures (torn) Click Pallial line Location on shell where mantle attaches Nacre/Mother of pearl Iridescent layer of the shell, pearls form here

10. Identify the following Structures Click Labial pulps (4) Two on each side of the mouth, used to sort food and move it toward the mouth Muscular Foot Used for movement, appears shrunken in preserved clam Gills (respiratory) Used to remove oxygen from the water Mantle Produces the shell Mantle Produces the shell

11. Identify the following Structures Click Gills have feather- like appearance, usually two on each side Foot Visceral mass Thick area above foot, digestive glands, intestines and gonads located here Mantle Visceral mass Foot Top View

12. Identify the following Structures Click Gills, mantle and l abial pulps removed Visceral mass with outer layer removed Digestive glands (usually green) produces enzymes to digest food Gonads (usually yellowish/white) reproductive function Tube-like intestines wind through the visceral mass

13. Identify the following Structures Click Gills, mantle, outer covering of visceral mass and l abial pulps removed Heart (exposed by removing protective membrane) Each specimen may appear a little different, damaged adductor muscle shown here (could indicate some type of disease or condition that attacked the muscle)

14. Dissection 101: Clam Produced by Dakota State University and South Dakota Public Broadcasting