1. Real-time multi-parameter spectroscopy and localization in three-dimensional single-particle tracking by Christian Hellriegel, and Enrico Gratton Interface Volume 6(Suppl 1):S3-S14 February 6, 2009 ©2009 by The Royal Society

2. Schematic of experimental setup. Christian Hellriegel, and Enrico Gratton J. R. Soc. Interface 2009;6:S3-S14 ©2009 by The Royal Society

3. Modelled modulation versus distance lookup tables. Christian Hellriegel, and Enrico Gratton J. R. Soc. Interface 2009;6:S3-S14 ©2009 by The Royal Society

4. (a–c) Retrieved positions from an immobile nanoparticle embedded in a sol-gel glass. Christian Hellriegel, and Enrico Gratton J. R. Soc. Interface 2009;6:S3-S14 ©2009 by The Royal Society

5. An immobile nanometre-sized luminescent bead has been moved along a specified path in three dimensions. Christian Hellriegel, and Enrico Gratton J. R. Soc. Interface 2009;6:S3-S14 ©2009 by The Royal Society

6. Latex beads (100 nm) in glycerine. Christian Hellriegel, and Enrico Gratton J. R. Soc. Interface 2009;6:S3-S14 ©2009 by The Royal Society

7. Sequence of spectra from a mixture of labelled beads passing through the observation volume. Christian Hellriegel, and Enrico Gratton J. R. Soc. Interface 2009;6:S3-S14 ©2009 by The Royal Society

8. (a,b) Image sequence showing a cross section of a cellular membrane. Christian Hellriegel, and Enrico Gratton J. R. Soc. Interface 2009;6:S3-S14 ©2009 by The Royal Society

9. Trajectories of uPAR aggregates near the cell membrane. Christian Hellriegel, and Enrico Gratton J. R. Soc. Interface 2009;6:S3-S14 ©2009 by The Royal Society