1. THE KREB’S CYCLE What Goes Around Comes Around

2. The Krebs Cycle The Krebs Cycle What Goes Around Comes Around Part 1: Preparation of Pyruvate Pyruvate (3C) Acetyl – Coenzyme A Mitochondria Cytosol Mitochondria Outer Membrane CO 2 H NAD+ NADH Coenzyme A (2C) Acetic Acid Transport Protein

3. Acetyl CoA (2C) Oxaloacetate (4C) CoEnzyme A Citrate (6C) H2OH2O Isocitrate (6C) CO 2 NAD+ NADH a-Ketoglutarate (5C) CoEnzyme A CO 2 NAD+ NADH Succinyl- CoA (4C) CoEnzyme A GTP GDP ADP ATP FAD+ FADH H2O Succinate (4C) Fumarate (4C) Malate (4C) Part 2: Kreb’s Cycle TOTALS ATP = NADH = FADH = CO 2 = 1 X 2 = 2 4 X 2 = 8 THE KREB’S CYCLE 1 X 2 = 2 3 X 2 = 6 Kreb’s Cycle Song

4. Slide 2

5. CHORUS The Krebs Cycle Acetyl CoA combines with oxaloacetic acid To create a citric acid. That's how it begins. Then a water removed and added makes it isocitric acid, Which, in turn, oxidized and loses CO2. Then alpha-ketoglutaric is oxidized again, Losing carbon dioxide with an NADH spin. Then through a phosphorylation Succinyl CoA congeals a GDP to GTP to toss back CoASH. CHORUS: Acetyl CoA, citric acid, iso-, Alpha-ketoglutaric, succinyl CoA, Succeeded by succinic, fumaric and malic, Then oxaloacetic starts the cycle again. Soon we see succinic acid forming a fumaric acid As it forms an FADH2 along the way. Water washed upon fumaric makes it into acid malic. Now there's only one step left, so let's be on our way. With one more oxidation, resisting degradation, A final NADH is made and brings us to the end. Oxaloacetic acid, first and last, a simple acid Then accepts an acetyl CoA and starts again. melody and lyrics by Lynda Jones; adapted by Do Peterson and Greg Crowthe Greg: geek vocals, keyboard Lori: lead vocals Kirk L. Van Scoyoc: banjo Do: bass, hyper-dramatic vocals, keyboard, acoustic and electric guitar, drum OXIDATION