1. Real-time Monitoring technology of Mass spectrometry
Speaker : Achong

2. Outline IMS PTR-MS SIFT-MS Application of IMS IMS-TOF IMS-IMS-TOF
Application of PTR-MS
PIT-MS

3. Real-time Monitoring Mass spectrometry
Environmental science
Biochemical and medical diagnosis
Workplace safety
Security check

4. EI source
Analysis of atmospheric air is especially challenging using electron ionization mass spectrometry, because the gas loading of the ion source by N2 and O2 (as well as water vapor) can be excessive.
70 ev

5. Real-time Monitoring MS
Soft chemical ionization
Measurement in Real-Time
Without pre-concentration system

6. I. IMS (ion mobility spectrometer)
Ion mobility mass spectrometry (Eiceman,1991; Baumbach, 2001)
Analytical Chemistry, A390-A397, November 1, 2004

7. Ion mobility (K)
Journal of Bimolecular Technique, Vol.13, Issue 2, June, 2002

8. Ion Mobility-Mass Spectroscopy
Witt & Bowers: JACS. 2000, 122, 3458.

9. Ion mobility mass spectrometry
Advantages:
Multiform ion source could be applied.
Some isomer could be separated.
Real-time measurement
Disadvantages:
Lower mass resolution
Lower sensitivity to allow accurate analysis of complex mixtures.
Standard is requisite

10. II. PTR-MS (proton-transfer reaction)
Proton-transfer reaction-mass spectrometry (Lagg A, Taucher J, Hansel A, Lindinger W )

11. proton-transfer reaction
H3O+ + R → RH+ + H20
[RH+]
= [H3O+]o(1 - e-k[R]t)
= [H30+]o[R]kt
J. Mass Spectrom Ion Processes, 173 (1998)

12. J. Mass Spectrom Ion Processes, 173 (1998) 191-241

13. J. Mass Spectrom Ion Processes, 173 (1998) 191-241

14. PTR mass spectrometry Advantages:
Soft ionization and low matrix effect
Real-time measurement
Low limit of detection (0.5 ppbv for 1-min measurements)
Disadvantages:
A large number of VOCs could not be identified and
quantified.

15. III. SIFT-MS (selected ion flow tube)
Adams NG, Smith D
Spanel P ,Smith D

17. SIFT mass spectrometry
Advantages:
application of multiple reagent ions (H3O+, NO+ and O2+)
quantification in real-time
discriminate isomers in Real-Time
Disadvantages:
The kinetic parameters of the reagent ion and the analyte ion must be known.
Selected ion flow tube mass spectrometry for no-line trace gas analysis
David Smith and Patrik Spanel
Reference : Mass Spectrometry Reviews, 2005, 24, 661– 700

18. Application of IMS
IMS/TOF
IMS/IMS/TOF

19. Ion Mobility/TOFMS
Anal. Chem. 2003, 75,

20. Pulse sequence of IMS & TOF
Anal. Chem. 2003, 75,

21. example spectrum
Analytical Chemistry, 1998 Vol. 70, No. 11, June 1,

22. Anal. Chem. 2003, 75,

23. collision-induced dissociation

24. IMS/IMS/TOF
Anal. Chem. 2006, 78,

25. operational mode A
Anal. Chem. 2006, 78,

26. operational mode B
Anal. Chem. 2006, 78,

27. operational mode C
Anal. Chem. 2006, 78,

28. Application of PTRMS
PIT-MS

29. PIT-MS

30. PIT-MS proton-transfer ion trap-mass spectrometry
J Am Soc Mass Spectrom 2005, 16, 1316–1324

31. CID process on ion-trap MS

32. calibration measurement of the PIT-MS
J Am Soc Mass Spectrom 2005, 16, 1316–1324

33. single CID measurement of m/z 59
ambient air
pure acetone
pure propanal
J Am Soc Mass Spectrom 2005, 16, 1316–1324

34. CID of propanal and acetone
m/z=59
m/z=41
m/z=31
J Am Soc Mass Spectrom 2005, 16, 1316–1324