1. CHAPTER 25 CAMPBELL and REECE

2. Conditions on early Earth made the Origin of Life possible Macroevolution : evolutionary change above the species level examples: 1. emergence of terrestrial vertebrates 2. mass extinctions impact on diversity of life 3. origin of key adaptations like flight in birds

3. Where did 1st cell come from? 4 main stages could have produced very simple cells: 1. The abiotic synthesis of small organic molecules 2. Joining of these small molecules into macromolecules (proteins, nucleic acids) 3. Packaging of these macromolecules into protocells, droplets with membranes that maintained internal chemistry different from their surroundings 4. Origin of self-replicating molecules that eventually made inheritance possible

4. Synthesis of Organic Cpds on early Earth Planets of our solar system formed ~ 4.6 billion yrs ago 1 st few hundred million yrs conditions would not have allowed life on Earth

5. 1 st Atmosphere Collisions would have vaporized any water preventing seas from forming Atmosphere thick with gases released from volcanic activity

6. 1st Atmosphere 1920’s: Oparin (Russian chemist) and Haldane (British scientist) each came to conclusion early atmosphere was reducing environment (gain e-) in which organic compounds could have formed from simpler molecules

7. 1 st Organic Compounds Energy sources: Lightning Thermal energy Intense UV radiation

8. Primordial Soup Haldane had hypothesized the early seas site of 1 st organic compounds  1 st cells Miller & Urey (Univ. of Chicago) in 1950’s Tested Oparin & Haldane ‘s premise Created a reducing atmosphere Added compounds considered to have been found existing on early earth (hydrogen gas – H2, ammonia – NH3, methane – CH4, and H2O vapor)

9. Miller & Urey Experiment: In 1953, Stanley Miller set up a closed system to simulate conditions thought to have existed on early Earth 1. Water mixture in “sea” flask was heated; vapor entered atmosphere flask 2. “Atmosphere” flask contained mix of hydrogen gas, methane, ammonia, and water vapor (believed to mimic early Earth’s atmosphere) 3. Sparks were discharged to mimic lightning 4. Condenser cooled the “atmosphere”, “raining” water and any dissolved molecules down into sea flask 5. As material cycled through apparatus, Miller periodically collected samples for analysis Results: Miller identified variety of organic molecules common in organisms (including simple molecules like formaldehyde (CH2O) and hydrogen cyanide (HCN) and more complex molecules (like amino acids and hydrocarbons) Conclusion: Organic molecules (a 1st step in the origin of life) may have been synthesized abiotically on early Earth

10. Miller & Urey’s Experiment

11. Miller & Urey’s Results

12. Miller-Urey Experiment Clip

13. Miller & Urey’s Results Have been repeated using same or similar ingredients, different recipes for the atmosphere and they also produced organic compounds Still ?s about amounts of methane, ammonia (was there really enough to make it a reducing environment?) Some repeated experiment in non-reducing, non- oxidizing conditions & still produce organic compounds

14. Miller-Urey Experiment demonstrates: 1. Abiotic synthesis of organic molecules is possible under various assumptions about the composition of Earth’s early atmosphere 2. Meterorites may also have been source of minerals and organic molecules Contain amino acids, lipids, simple sugars, uracil

15. Murchison Meteorite

16. Fell to Earth in so named town in Australia in 1969 large (100 kg) and was quickly retrieved 2010 article published in Scientific American: results of mass spectrometry (separating compounds based on charge & size) have revealed at least 14,000 unique molecules

17. Abiotic Synthesis of Macromolecules 2009 study showed the abiotic synthesis of RNA monomers can occur spontaneously from simpler precursor molecules Drip solutions with amino acids (aa) or RNA nucleotides onto hot sand, rock, or clay  polymers of aa & RNA (w/out using enzymes or ribosomes)

18. Protocells (Protobionts) Basic characteristics of life : reproduction & metabolism: So 1 st cells would have had to be able to reproduce which would have required them to have a source of nitrogenous bases, sugars, phosphate groups Now complex enzymes make this all happen

19. Vesicles as 1 st step? When lipids & other organic molecules added to water  vesicles spontaneously form lipid bilayer (separation of hydrophilic & hydrophobic molecules) These abiotically produced vesicles “reproduce” and grow on their own. Clay, like from early Earth will be absorbed into the vesicles some vesicles demonstrate semi- permeability

20. Self-Replicating RNA RNA (when folded)can act as enzyme RNA catalysts called: ribozymes Some can make complimentary strands of short pieces of RNA  mutations  more stable &/or successful

21. Ribozyme Once self-replicating RNA possible much easier for further changes to happen. Once double-stranded DNA appeared it would have been more stable so RNA left with role we see today