1. TrueAllele ® Challenges in Court and Culture Cybergenetics © 2003-2012 9 th Annual DNA Technology Educational Seminar Centre of Forensic Sciences and the Promega Corporation Toronto, Ontario November, 2012 Mark W Perlin, PhD, MD, PhD Cybergenetics, Pittsburgh, PA, USA

2. Commonwealth v. Foley Perlin MW, The Blairsville slaying and the dawn of DNA computing, in Death Needs Answers: The Cold-Blooded Murder of Dr. John Yelenic, Niapas A, New Kensington, PA: Grelin Press, 2012. Murder Victim's fingernails Minor 7% DNA mixture Crime: Evidence: Challenge: StatisticMethod 13 thousandinclusion 23 millionobligate allele 189 billionTrueAllele Defense: If mixtures are reliable, dont we expect the same information from all methods?

3. TrueAllele v. Inclusion information MW Perlin, A Sinelnikov. An information gap in DNA evidence interpretation. PLoS ONE, 2009.

4. Predict match information 15 loci 12 loci 67 information MW Perlin, A Sinelnikov. An information gap in DNA evidence interpretation. PLoS ONE, 2009.

5. Regina v. Broughton Arson, terror Fuse Low-level DNA mixture Crime: Evidence: Challenge: low template mixture three DNA contributors triplicate amplification post-PCR enhancement vWA locus

6. Likelihood ratio Genoype probabilility (vWA) Assume coancestry at a theta value of 1%, and that there are three contributors present. A match between Mr. Broughton and the fuse is 3,620,000 times more probable than a coincidental match to an unrelated Caucasian. 6x Perlin MW. Explaining the likelihood ratio in DNA mixture interpretation. Promega's Twenty First International Symposium on Human Identification, 2010; San Antonio, TX.

7. New York State Police validation papers Perlin MW, Legler MM, Spencer CE, Smith JL, Allan WP, Belrose JL, Duceman BW. Validating TrueAllele ® DNA mixture interpretation. Journal of Forensic Sciences. 2011;56(6):1430-47. Perlin MW, Belrose JL, Duceman BW. New York State TrueAllele ® Casework validation study. Journal of Forensic Sciences. 2013;58(6):in press.

8. Information: sensitivity MW Perlin, MM Legler, CE Spencer, JL Smith, WP Allan, JL Belrose, BW Duceman. Validating TrueAllele ® DNA Mixture Interpretation. Journal of Forensic Sciences, 2011. 7.03 7.03 6.24 6.24 13.26 Quantitative TrueAllele Inclusion method

9. New York State Forensic Science regulatory approval

10. Regina v. Duffy & Shivers Murder, terror Mobile phone, match stick Low DNA amount; mixtures Crime: Evidence: Challenge: Real IRA claimed responsibility March 7, 2009 Antrim, Northern Ireland

11. Combining DNA evidence a b c a b c a b a c singlespairsall threedata 102030 b c a b c 1.1 million 6.04 STR tests 40,000 4.60 26,000 4.42 560 2.75 25 1.40 27 1.43 6 0.75 MW Perlin, Combining DNA evidence for greater match information, American Academy of Forensic Sciences 64 th Annual Meeting, Atlanta, GA, February 24, 2012.

12. TrueAllele match statistics 5.91 trillion 6.01 billion 1.1 million Colin Duffy Brian Shivers Perlin MW, Galloway J. Computer DNA evidence interpretation in the Real IRA Massereene terrorist attack. Evidence Technology Magazine. 2012;10(3):20-23.

13. Admissibility ruling … In Broughton, Judge Eccles did not give a reasoned judgment explaining his decision. … I am satisfied that the stage has now been reached in the case of this system where it can be regarded as being reliable and accepted, and I am satisfied that Dr Perlin has given his evidence in a credible and reliable fashion. In the light of these conclusions I can see no basis on which I could properly exercise my discretion … to exclude this evidence, and I therefore admit it in evidence. December 1, 2011 The Honorable Mr. Justice Hart

15. Pennsylvania precedent Commonwealth v. Kevin Foley Pennsylvania Superior Court February 15, 2012

16. CPI information Information log(LR) Statistic log(mCPI)

17. Modified CPI information Information log(LR) Statistic log(mCPI) SWGDAM 2010: DNA mixture interpretation guidelines Interpretation of complex DNA mixtures must account for data uncertainty

18. TrueAllele and the crime lab Casework system Interpretation services

19. More information perlin@cybgen.com http://www.cybgen.com/information Courses Newsletters Newsroom Presentations Publications