1. EXPASY TOOLS

2. 2  The ExPASy (the Expert Protein Analysis System) World Wide Web server (http://www.expasy.org)  Provides access to scientific databases and software tools (i.e., resources) in different areas of life

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5. ELM

6. 6 Purpose Mainly focuses on annotation and detection of eukaryotic linear motifs (ELMs)

7. 7 I NPUT Protein sequence Enter Uniprot identifier or accession number: (auto-completion) e.g. EPN1_HUMAN, P04637, TAU_HUM ANEPN1_HUMANP04637TAU_HUM AN

8. 8 REESULT INTERPERTATION

9. PROTPARM

10. 10 Purpose: ProtParam computed parameters include the molecular weight, theoretical pI, amino acid composition, atomic composition, instability index, aliphatic index and grand average of hydropathicity.

11. 11 I NPUT Protein sequence File from laptop

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22. SulfoSite

23. Place your screenshot here 23 Purpose: SulfoSite is to computationally predict sulfation sites within given protein sequences. A representative set of 162 protein tyrosine sufation sites from the Swiss-Port were used to analyze characteristics and to train and test in SulfoSite

24. 24 I NPUT Protein sequences Directly Attached file from computer

25. 25 REESULT INTERPERTATION

26. Compute pI/Mw tool

27. Place your screenshot here 27 Purpose: Compute pI/Mw is a tool which allows the computation of the theoretical pI (isoelectric point) and Mw (molecular weight) for a list of UniProt Knowledgebase (Swiss- Prot or TrEMBL) entries or for user entered sequences.

28. 28 I NPUT UniProtKB/Swiss-Prot protein identifiers (ID) Protein sequence in single letter code. Upload a file from your computer

29. 29 REESULT INTERPERTATION

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32. Translate tool

33. Place your screenshot here 33 Purpose: Translate is a tool which allows the translation of a nucleotide (DNA/RNA) sequence to a protein sequence.

34. 34 I NPUT Enter a DNA or RNA sequence.

35. 35 REESULT INTERPERTATION

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37. 37 THANKS! Any questions? You can find me at zahrashahzadi1134@gmail.com