1. Introduction to Microvolumetrics and Pipetting
Lab 1
Introduction to Microvolumetrics and Pipetting

2. Micropipette Use Twist dial to desired volume
Add disposable pipette tip AND close the lid
Press plunger to first stop
Insert pipette tip into solution to be
transferred
Slowly release plunger to retrieve liquid
Move pipette tip into desired tube
Press plunger past first stop to second stop
to transfer liquid, keep the plunger down
as you remove it from the tube.
8. Eject tip

3. Micropipette Use 1. Twist dial to desired volume
. Add disposable pipette tip and close the lid
. Press plunger to first stop
. Insert pipette tip into solution to be
transferred
. Slowly release plunger to retrieve liquid
. Move pipette tip into desired tube
. Press plunger past first stop to second stop
to transfer liquid, watch liquid stick to wall
of tube. Remove tip, then release plunger.
8. Eject tip

4. - “1st stop” to take in - “2nd stop” to expel
liquid the liquid

5. Always hold micropipette and epitubes at eye level when drawing up liquids

6. YouTube Video Intro to micropipetting video clip:
Introduction to Gel Electrophoresis video clip:

7. Setting the Volume in a P20
10.0 μl μl

8. Practice 1 8 5 2 1 5 3 3
18.0 μl
5.2 μl
15.3 μl
3.0 μl

9. P20 Micropipette 2 Maximum setting = Minimum setting = 20.0 μl 2.0 μl2 2

10. Key to Video expensive, damage 8. Pipette tip Never, outside 9. Tip
1.52μl, 15.2 μl,
152 μl, 520 μl
4. First, down
5. Slowly
6. Expelled
7. before
8. Pipette tip
9. Tip
10. Volume limits
11. Tip
12. First stop
13. Controlled
14. Lay the pipette down
15. small, side

11. YouTube Video Intro to micropipetting video clip:
Introduction to Gel Electrophoresis video clip:

12. Laboratory Techniques: Using A Micropipette (review of diff sizes, showing ranges for each, uses same pipette we use, safe use review, tips, 1st & 2nd stops, 5:26)
The Micropipette and Micropipetting Technique (review, 3 wrences? Otherwise good, 3:08)
How to use a Micropipette (University lab, 1st & 2nd stops, new tips each time, check volumn setting, 2:20)
Mastering Micropipettes (University of Colorado, Biology lab, setting volume reveiw, adding tip, 1st & 2nd stops, proper time to release plunger, 3:30)

13. Electrophoresis Chamber

14. Loading Gels:
Insert pipette tip:
Under buffer (liquid) level
Above gel well

15. Micropipetting technique A

16. technique B
Close the tips!

17. Technique C
Billiard style

18. Micropipette tip should be ABOVE the well NOT IN IT!!!!

19. Micropipette tip punched right through the gel
See dye under the wells

20. NICE!

22.
Agarose Gel Electrophoresis (4:06, great overview, DNA neg charge, loading gel, pipette use overview, loading gels
Introduction to Gel Electrophoresis video clip: