1. Mapping of an allergenically important determinant of grass group I allergens 
Kara M. Hiller, MSa, Robert E. Esch, PhDb, David G. Klapper, PhDa 
Journal of Allergy and Clinical Immunology 
Volume 100, Issue 3, Pages (September 1997)
DOI: /S (97)70246-X
Copyright © 1997 Mosby, Inc. Terms and Conditions

2. Fig. 1
Inhibition ELISA with immobilized Gpl. Dilutions of STF, CGG-STF, ASN, and Gpl were tested for their ability to inhibit the binding of mAb to immobilized Gpl. Percent inhibition is expressed as a percentage of a control with no inhibitor.
Journal of Allergy and Clinical Immunology  , DOI: ( /S (97)70246-X)
Copyright © 1997 Mosby, Inc. Terms and Conditions

3. Fig. 2
Inhibition ELISA with immobilized grass extracts. Coated extracts are named next to the corresponding symbol, followed by inhibitors in parentheses. Grass extracts of MF, SV, PR, JU, TI, OR, and RT were coated onto a microtiter plate. STF was tested for its ability to inhibit mAb binding to these seven Gpl-containing extracts. As a negative control, an excess of peptide VDK was also tested against mAb for binding to all seven extracts. VDK showed negligible inhibition for all seven extracts, and this is represented here by a point. As a positive control, the ability of Gpl to compete with mAb for binding to the extracts was also assessed. Only the results for Fes e 1 inhibition are shown. Results for all other extracts were very similiar.
Journal of Allergy and Clinical Immunology  , DOI: ( /S (97)70246-X)
Copyright © 1997 Mosby, Inc. Terms and Conditions

4. Fig. 3
Standard ELISA with immobilized peptide. STF, ASN, CGG-STF, and KLH-CGG-STF, and LGK were coated onto a microtiter plate and probed with mAb Results are expressed as absorbance at wavelength 405 nm.
Journal of Allergy and Clinical Immunology  , DOI: ( /S (97)70246-X)
Copyright © 1997 Mosby, Inc. Terms and Conditions