2. Cell Fractionation
The process by which cells are broken up and the organelles are isolated to be investigated.

3. Make sure the 2 last boxes are larger than the others!
Step
Reason
Preparation
Homogenisation
Make sure the 2 last boxes are larger than the others!
Ultracentrifugation

4. Step Tissue placed in a… Cold Isotonic Buffered …solution Reason
Prevent enzyme activity
Prevent osmosis
maintain correct and constant pH

5. Fluid extracted (homogenate) and filtered Break cells up
Step
Tissue placed in a…
Blender or pestle
Fluid extracted (homogenate) and filtered
Reason
Break cells up
Remove whole cells and debris

6. Reason Step Homogenate placed into an ultracentrifuge
Spun at low speed (1000 x g)
Sediment in base, supernatant on top
Extract supernatant
Spun at medium speed (4000 x g)
Spun at high speed (16000 x g)
Reason
Create a density gradient
Collect nuclei in sediment
Collect remaining organelles
Collect mitochondria in sediment
Collect E.R, lysosomes and ribosomes.

9. Bingo! Draw a 3 x 3 grid and write in 9 of the following words:
Lysosome, endoplasmic reticulum, mitochondria, golgi apparatus, ribosomes, enzymes, nuclei, isotonic, buffered, cold, homogenate, low speed, high speed, ultracentrifugation, cell fractionation,