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The Neuron Identity Problem: Form Meets Function
Gord Fishell, Nathaniel Heintz Neuron Volume 80, Issue 3, Pages (October 2013) DOI: /j.neuron Copyright © 2013 Elsevier Inc. Terms and Conditions
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Figure 1 Genetic Targeting of CNS Cell Types: The Sim1 BAC-Cre Driver Line KJ18 Provides Genetic Access to Corticostriatal Projection Neurons Developmental Cre expression is revealed by the recombination of the Rosa26_CAG-tdTomato Ai9 reporter line (shown in red). Adult pyramidal neurons expressing Cre activity is apparent after Cre-dependent AAV2/1.CAG.FLEX.EGFP.WPRE.bGH expression vector injections into primary motor cortex (shown in green) (Gerfen et al., 2013). Neuron , DOI: ( /j.neuron ) Copyright © 2013 Elsevier Inc. Terms and Conditions
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Figure 2 Schematic Showing the Progressive Steps Neural Progenitors Go Through in Order to Attain a Stable Cell Identity As neurons move from the proliferative zone to become integrated into circuits, they go through three successive steps: (1) initial specification in the neuroepithelium, (2) establishment of a ground-state identity upon becoming postmitotic, and (3) refinement of cell characteristics by local cues as they establish their connectivity. Neuron , DOI: ( /j.neuron ) Copyright © 2013 Elsevier Inc. Terms and Conditions
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Figure 3 Comparative Analysis of TRAP Data Reveals Cell-Type-Specific Translational Profiles A heat map showing the normalized expression of the top 100 ranked probesets from each sample across all samples. Note the blocks of genes detected as specific to each cell type (such as Pcp2) (Doyle et al., 2008). Neuron , DOI: ( /j.neuron ) Copyright © 2013 Elsevier Inc. Terms and Conditions
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