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Ingeborg Friehs, MD, Christof Stamm, MD, Hung Cao-Danh, PhD, Francis X

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Presentation on theme: "Ingeborg Friehs, MD, Christof Stamm, MD, Hung Cao-Danh, PhD, Francis X"— Presentation transcript:

1 Insulin-like growth factor-1 improves postischemic recovery in hypertrophied hearts 
Ingeborg Friehs, MD, Christof Stamm, MD, Hung Cao-Danh, PhD, Francis X. McGowan, MD, Pedro J. del Nido, MD  The Annals of Thoracic Surgery  Volume 72, Issue 5, Pages (November 2001) DOI: /S (01)

2 Fig 1 Glucose uptake measured as accumulation of 2-Deoxyglucose-6-phosphate (2-DG-6-P) by 31P-NMR spectroscopy over a period of 30 minutes in hypertrophied hearts (Hyper), age-matched nonhypertrophied control hearts (Control), insulin-like growth factor-1-perfused hypertrophied hearts (Hyper+IGF), and IGF-1+wortmannin-perfused hypertrophied hearts (Hyper+IGF/wort). n = 6/group, *p ≤ 0.05 versus Hyper+IGF and Control. All solutions contained a constant amount of insulin (10 IU/L). The Annals of Thoracic Surgery  , DOI: ( /S (01) )

3 Fig 2 Developed pressure measured 30 minutes after reperfusion (expressed as percent of preischemic values) in hypertrophied hearts (Hyper) with and without insulin-like growth factor-1 (Hyper + IGF-1) perfusion, with IGF-1+wortmannin (Hyper+IGF/wort) containing perfusate, and age-matched nonhypertrophied control hearts. n = 6/group, *p ≤ 0.05 versus IGF-1 perfused hypertrophied hearts and age-matched control hearts (Control). The Annals of Thoracic Surgery  , DOI: ( /S (01) )

4 Fig 3 (A) Representative blot of phosphatidylinositol-3-kinase (PI-3-kinase) activity in antiinsulin-receptor-substrate-1 immunoprecipitates from left ventricular muscle extracts of hypertrophied hearts perfused with Krebs-Henseleit (KH) solution containing insulin only or KH containing insulin+insulin-like growth factor-1 (IGF-1) compared with hearts from age-matched control animals perfused with KH containing insulin or insulin/wortmannin. The radioactivity at the origin is caused by [32P] ATP residues and other 32P-labeled materials. PIP indicates the position of migration of PI-3-P standard. (B) Summary of densitometry values expressed as arbitrary densitometry units for the three groups. *p ≤ 0.05; versus IGF-1-perfused hypertrophied hearts and age-matched control. The Annals of Thoracic Surgery  , DOI: ( /S (01) )

5 Fig 4 (A) Representative Western immunoblot of phosphatidylinositol-3-kinase (PI-3-kinase) protein of antiinsulin-receptor-substrate-1 immunoprecipitates from left ventricular muscle extracts from hypertrophied hearts perfused with Krebs-Henseleit (KH) solution containing insulin only or KH containing insulin and insulin-like growth factor-1 (IGF-1) compared with hearts from age-matched control animals perfused with KH containing insulin. (B) Summary of densitometry values expressed as arbitrary densitometry units for the three groups. There is no significant difference of PI-3-kinase protein content in any group. The Annals of Thoracic Surgery  , DOI: ( /S (01) )

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