1. G-CSF neutralization is protective in malaria.
G-CSF neutralization is protective in malaria. (A) Parasitemia of infected mice (n = 10) measured by counting thin blood smears. (B) Absolute counts of circulating neutrophils determined by FACS. Neutrophils were labeled with an anti-Ly6G antibody (n = 10). (C) NETs (MPO/DNA complexes) in plasma quantified by ELISA (n = 10). (D) Neutrophil liver infiltrates quantified by analysis of calgranulin A immunofluorescence in liver sections (n = 6). (E) Parasite sequestration in the liver (n = 10), measured by luminescence quantification of a luciferase-expressing strain of P. chabaudi. (F) Concentration of AST in plasma of mice at peak parasitemia (n = 10). Data are presented as mean ± SEM; each dot represents one biological replicate. Asterisks indicate significance: *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < by Kruskal-Wallis test. (G) G-CSF concentration in plasma of uncomplicated patients with malaria (n = 43) and healthy individuals (n = 9) in Gabon. Data are presented as mean ± SEM. Asterisks indicate significance: *P < 0.05, **P < 0.01, ***P < by Welch’s t test.
Sebastian Lorenz Knackstedt et al. Sci. Immunol. 2019;4:eaaw0336
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