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Determination of Per2 (left), MTNR1A (middle), and ERβ (right) protein expression levels in mammary tissues of rats sacrificed at ZT12. Determination of.

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Presentation on theme: "Determination of Per2 (left), MTNR1A (middle), and ERβ (right) protein expression levels in mammary tissues of rats sacrificed at ZT12. Determination of."— Presentation transcript:

1 Determination of Per2 (left), MTNR1A (middle), and ERβ (right) protein expression levels in mammary tissues of rats sacrificed at ZT12. Determination of Per2 (left), MTNR1A (middle), and ERβ (right) protein expression levels in mammary tissues of rats sacrificed at ZT12. A, Western blot results with whole tissue protein. β-Actin was used as an internal control. B, quantitative analysis of protein expression levels. Mean expression levels were calculated from densitometric intensities of bands determined with Western blot and normalized to β-actin. *, P < 0.05, significant difference. C, representative immunohistochemical staining on frozen sections of mammary tissues (×200; bar, 0.1 mm). a-c, NMU, methylselenocysteine diet; d-f: NMU, control diet; g-i: representative epithelial cells with positive staining are indicated by arrows and magnified photomicrograph (×400). Ming Zhu Fang et al. Cancer Prev Res 2010;3: ©2010 by American Association for Cancer Research

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