1. Quantitative PCR (A) and Western blot (B) analyses for expression of CD133 and ALDH isoforms in CD133+ and CD133− cells sorted from HCC cell lines PLC8024 and Huh7.
Quantitative PCR (A) and Western blot (B) analyses for expression of CD133 and ALDH isoforms in CD133+ and CD133− cells sorted from HCC cell lines PLC8024 and Huh7. ALDH1A1, along with several other ALDH isoforms, was found to be preferentially expressed in the CD133+ subpopulation. C. Quantitative PCR analysis for expression of ALDH1A1 in a panel of liver cell lines. Expression of ALDH1A1 in the liver cell lines directly correlated with their expression of CD133. D. Representative flow cytometry analyses of hepatoblastoma cell line HepG2 and HCC cell lines PLC8024 and Hep3B for ALDH activity using the ALDEFLUOR Kit (Stem Cell Technologies). Left, negative controls when the cells were treated with the ALDH inhibitor diethylaminobenzaldehyde (DEAB). SSC-H, side scatter; FL1-H, FITC channel or ALDH activity. Those cells gated in R2 represent the subpopulation of HCC cells that are positive for ALDH activity.
Stephanie Ma et al. Mol Cancer Res 2008;6:
©2008 by American Association for Cancer Research