1. Tumor spheroid induces mesothelial cell migration.
Tumor spheroid induces mesothelial cell migration. A, temporal analysis of adhesion dynamics of paxillin-GFP–labeled LP9 human peritoneal mesothelial cells incubated with or without RFP-actin–expressing OVCA433 cancer spheroids. Events of adhesion assembly and disassembly were scored. Arrows indicate adhesion disassembly. Scale bar, 5 μm. B, bar graph shows the ratio of adhesion assembly and disassembly events within the same area. A total of 150 assembly and disassembly events were analyzed per condition. C, GFP-labeled lung mesothelial cells were mixed at 1:500 ratio with nonlabeled lung mesothelial cells. Images show temporal behavior of single mesothelial cells in the presence or absence of cancer spheroids. Arrows indicate mesothelial cell movement away from intercalating spheroid. Scale bar, 100 μm. D, migration distance of single mesothelial cells was measured in the presence and absence of OVCA433 cancer spheroids. A total of 50 GFP-labeled mesothelial cells were analyzed per condition.
Marcin P. Iwanicki et al. Cancer Discov 2011;1:
©2011 by American Association for Cancer Research