1. AR promotes DNA double-strand break resolution.
AR promotes DNA double-strand break resolution. A, C4-2 cells were cultured in hormone-proficient media for 24 hours then treated as indicated. At indicated time points, cells were pretreated with BrdUrd for 2 hours and then fixed. Cells were incubated with BrdUrd antibody and propidium iodide and processed for fluorescence-activated cell sorting analysis. Data is graphed as normalized to untreated control. B and C, C4-2 cells were cultured in hormone-proficient media for 24 hours then treated as indicated. Cells were fixed at the indicated time points, stained with α-γ-H2AX and α-53BP1 antibodies, and imaged by confocal microscopy. Foci were counted and plotted as average foci per cell set relative to control at each time point. D, C4-2 cells were cultured in hormone-proficient, hormone-deficient, or hormone-deficient supplemented with 0.1 nmol/L DHT media for 24 hours then treated with 15 Gy ionizing radiation and harvested at indicated time points. Single-cell gel electrophoresis was conducted and tail moments assessed. *, P < 0.05; **, P < 0.01.
Jonathan F. Goodwin et al. Cancer Discovery 2013;3:
©2013 by American Association for Cancer Research