1. Effect of dexamethasone on PCDGF/GP88 mRNA and protein expressions and effect of PCDGF/GP88 on dexamethasone-induced cell death.
Effect of dexamethasone on PCDGF/GP88 mRNA and protein expressions and effect of PCDGF/GP88 on dexamethasone-induced cell death. ARP-1 cells were seeded at 4 × 104/mL in RPMI 1640 supplemented with 10% charcoal-treated FBS and treated with different concentrations of dexamethasone. A, dose response of dexamethasone (Dex) effect on PCDGF/GP88 mRNA expression after 24-hour treatment as measured by Northern blot. Top, PCDGF/GP88 mRNA expression. Bottom, 28S rRNA expression to indicate equal RNA loading. B, effect of dexamethasone on PCDGF/GP88 protein expression. Western blot analysis was carried out to measure PCDGF/GP88 expression levels. Top, PCDGF/GP88 protein expression. Bottom, β-actin protein expression to indicate equal loading. Con, control. C, after 48-hour treatment, effect of PCDGF/GP88 on dexamethasone-induced death as measured by live cell density. D, after 48-hour treatment, effect of PCDGF/GP88 on dexamethasone-induced cell death as measured by percentage survival. Columns, mean; bars, SD. *, P < 0.05, compared with 10−7 mol/L dexamethasone-treated sample (second column), t test.
Wengang Wang et al. Clin Cancer Res 2006;12:49-56
©2006 by American Association for Cancer Research