1. Silencing hOGG1 triggers caspase-3 and caspase-7 activation in response to H2O2 in GM00637 cells.
Silencing hOGG1 triggers caspase-3 and caspase-7 activation in response to H2O2 in GM00637 cells. A. Cells were treated with 50 μmol/L H2O2 for 1 h. The activated (cleaved) caspase-3 and caspase-7 were detected by Western blot analysis using specific antibodies for caspase-3 and caspase-7 at the indicated time points after the H2O2 treatment. Data were normalized with α-tubulin expression. B. After control-siRNA– and hOGG1-siRNA–transfected GM00637 cells were treated with or without 60 μmol/L caspase inhibitor and the indicated concentration of H2O2, caspase-3/7 activities were analyzed using Promega Caspase Glo 3/7 kit as described in Materials and Methods. Data were plotted as the relative units of luciferase intensity of three separate experiments. Points, mean; bars, SD. C. Cell viability was determined by trypan blue exclusion under the same experimental condition as in B. The extent of cell death was estimated as the percentage of total cell number that remained unstained. The control-siRNA–transfected cells served as experimental controls in this study. Points, mean from five separate experiments; bars, SD.
Cha-Kyung Youn et al. Mol Cancer Res 2007;5:
©2007 by American Association for Cancer Research