1. Eye piece
Eye piece - magnifies objects
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2. Arm
Support arm - holds the lenses in position over the stage

3. Low Power Objective
Low Power Objective – a small lens with low magnifying power.

4. Clips - to secure specimen slide to the stage
Stage Clips

5. Coarse focus adjustment
Coarse focus dial - for focusing and large movements of the stage

6. Fine Focus
Adjustment
Fine focus dial - for focusing and small movements of the stage

7. Revolving nose piece
Revolving nose piece - for changing objective lenses

8. High Power Objective
High-Power objective – A large lens with high magnifying power.

9. Function
Stage
Stage - for supporting specimen slide
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10. Diaphragm dial
Diaphragm dial - adjusts the amount of light that shines on to the slide

11. Aperture- hole or an opening through which light travels

12. Light/ mirror
This directs the light upward onto the slide.

13. Base
Base - weighted to keep microscope stable

14. Guidelines for Using the Light Microscope
Microscopes are expensive precision instruments and must be handled with care.
Use only the supporting arm of the microscope to lift it.
Always carry the microscope with one hand underneath the base and the other hand holding the supporting arm.
Keep the instrument in a box or under a cover when not in use.
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15. Guidelines for Using the Light Microscope
Never touch the lenses with your fingers.
Keep the lenses clean by wiping them with lens tissue.
Always handle microscope slides by their edges.
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16. Total Magnification
The total magnification of an image is equal to the magnifying power of the eye piece lens multiplied by the magnifying power of the objective lens.
Eye piece
Objective lens
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17. Total Magnification
The magnifying power of each lens is usually inscribed on the lens barrel.
Eye piece
For example, if a ×10 objective lens is used, assuming the eye piece magnification is ×10, the total magnification of the image will be (×10) × (×10) = × 100.
Objective lens
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18. Selecting the Lowest Power Objective Lens
It is normal practice to look at a specimen using the lowest available magnification first, then to look more closely at the specimen using a higher magnification.
To do this, position the ×4 objective lens so that it lies directly above the hole in the stage.
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19. Placing the Slide on the Stage
Before you place a slide on the stage, use the coarse focus dial to position the stage of the microscope so that there is at least a 2 cm gap between the objective lens and the stage.
Place the slide on the stage, so that the specimen is over the middle of the hole on the stage.
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20. Placing the Slide on the Stage
Make sure that light is shining through the slide, and then secure it in place with the clips.
This will help to prevent the slide from being dislodged when you are viewing it.
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