1. SLC30A10 protects DAergic neurons from neurodegeneration induced by Mn exposure.
SLC30A10 protects DAergic neurons from neurodegeneration induced by Mn exposure. A–E, Worms expressing dat-1::GFP were synchronized in the L1 stage and treated with 0 mm Mn (A) or 10 mm Mn for 1 h (B–E). DAergic neurons were then imaged as described in Materials and Methods. In B–E, arrowheads indicate degeneration in neuronal processes, and asterisks indicate degeneration in cell bodies. Scale bars, 10 μm. F–H, Worms expressing dat-1::GFP only (control) or dat-1::GFP along with dat-1::SLC30A10–WT or L89P were grown, treated with 0, 10, or 25 mm Mn, and imaged as described above. For each genotype, the number of worms in which DAergic neurons were “intact” (i.e., did not show any evidence of neurodegeneration) was calculated as described in Materials and Methods (mean ± SE; n = 3 experiments with at least 20 worms per Mn treatment condition, per genotype, per experiment; *p < 0.05, **p < 0.01, and ***p < for the difference between control and other transfection conditions by one-way ANOVA, followed by Tukey–Kramer post hoc test).
Dinorah Leyva-Illades et al. J. Neurosci. 2014;34:
©2014 by Society for Neuroscience