1. HLA-A*0201 restriction of SSX2-derived p103-111 stimulated CD8+ T-cells.
HLA-A*0201 restriction of SSX2-derived p stimulated CD8+ T-cells. (A) COS7/A2 cells were loaded with decreasing concentrations of SSX2 p peptide (black) ranging from 2 to µg/ml. For blocking, the anti-pan human MHC-I antibody (clone W6/32) (grey) or the anti-HLA-A*02 antibody (clone BB7.2) (white) was added. (B) To displace SSX2 p , T2 cells were loaded with a mix of SSX2 p peptide (0.02 µg/ml) and increasing concentrations of the HLA-A*0201 binding, CMV-derived p peptide. Reactions were measured by IFN-gamma ELISPOT assay.
Claudia Wagner et al. Cancer Immun 2003;3:18
Copyright © 2003 by Michael Pfreundschuh