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The ACIN1 Gene is Hypermethylated in Early Stage Lung Adenocarcinoma

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Presentation on theme: "The ACIN1 Gene is Hypermethylated in Early Stage Lung Adenocarcinoma"— Presentation transcript:

1 The ACIN1 Gene is Hypermethylated in Early Stage Lung Adenocarcinoma
Yujian Shu, MD, Tatsuo Iijima, MD, Weihong Sun, MD, Junko Kano, MD, Tadashi Ishiyama, MS, Chigusa Okubo, MS, Yoichi Anami, MD, Ryota Tanaka, MD, Shimao Fukai, MD, Masayuki Noguchi, MD  Journal of Thoracic Oncology  Volume 1, Issue 2, Pages (February 2006) DOI: /S (15)31532-X Copyright © 2006 International Association for the Study of Lung Cancer Terms and Conditions

2 FIGURE 1. a: Phase-contrast microphotograph of PL16T which is a immortalized cell line derived from atypical adenomatous hyperplasia. (original magnification x100) b: Phase-contrast microphotograph of PL16B which is a immortalized cell line derived from the resected end of bronchus of the same patient. (original magnification x100) c: Histological feature of small-sized adenocarcinoma with bronchioloalveolar carcinoma component. (case 5 in table 2) (original magnification x4) d: Histological feature of small-sized adenocarcinoma without bronchioloalveolar carcinoma component. (case 24 in table 2) (original magnification x4) Journal of Thoracic Oncology 2006 1, DOI: ( /S (15)31532-X) Copyright © 2006 International Association for the Study of Lung Cancer Terms and Conditions

3 FIGURE 2. Genome structure of the ACIN1 gene and the locations of CpG islands (full black boxes). The position of the DNA fragment isolated by MCA-SSH is indicated by the open box. C denotes the cytosine residues at the 5′: end of the isolated DNA fragment, with unknown methylation status. During sodium bisulfite DNA modification, unmethylated cytosine residues are converted to thymine, whereas methylated cytosine residues are retained as cytosine at the CpG site. After bisulfite DNA modification, the DNA fragments were amplified by PCR using appropriate primers and their sequences were determined with an automated sequencer. Journal of Thoracic Oncology 2006 1, DOI: ( /S (15)31532-X) Copyright © 2006 International Association for the Study of Lung Cancer Terms and Conditions

4 FIGURE 3. Expression of the ACIN1 gene. Expression levels were analyzed by quantitative RT-PCR in the pair of cell lines (PL16T and PL16B), in three surgical specimens of lung adenocarcinoma with BAC component (case numbers 11, 13, 21; see table 2) and in three specimens of adenocarcinoma without BAC component (case numbers 25, 26, 27; see table 2) together with their normal adjacent tissues. T with BAC: adenocarcinoma with bronchioloalveolar carcinoma component; T without BAC: adenocarcinoma without bronchioloalveolar carcinoma component. Journal of Thoracic Oncology 2006 1, DOI: ( /S (15)31532-X) Copyright © 2006 International Association for the Study of Lung Cancer Terms and Conditions

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