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Histone modification status at the Tβ4 promoter and at the protein level in the HuH7 sublines. Histone modification status at the Tβ4 promoter and at the.

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Presentation on theme: "Histone modification status at the Tβ4 promoter and at the protein level in the HuH7 sublines. Histone modification status at the Tβ4 promoter and at the."— Presentation transcript:

1 Histone modification status at the Tβ4 promoter and at the protein level in the HuH7 sublines.
Histone modification status at the Tβ4 promoter and at the protein level in the HuH7 sublines. A, Schematic representation of the genomic structure of the Tβ4 gene for ChIP assay. Boxes denote the exons of Tβ4. The three double-headed arrows indicate the regions examined by ChIP assay. B, Histone modification status in HuH7-F0 and HuH7-F12 cells. ChIP assay was conducted by using antibodies against active (H3K4me3 and H3K27ac) and repressive (H3K9me3 and H3K27me3) histone remarks at the three regions CP1, CP2, and CP3. Representative images and semiquantification measurements are displayed. C, Quantitative ChIP analysis of Tβ4 at the region CP2 in HuH7-F0, HuH7-F6, and HuH7-F12 cells. Bars are the mean ± SE. *, P < 0.01 by ANOVA with Dunnett post hoc test. D, Immunoblots of H3K4me3 and H3K27ac. Yoshiteru Ohata et al. Mol Cancer Ther 2017;16: ©2017 by American Association for Cancer Research

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