1. High and maximum containment laboratories

2. Outline Achieving Containment NIOH ASPIRE Facility NICD BSL4 Facility
Containment Barriers
HVAC systems and Control
Architectural components
Sterilisation systems
NIOH ASPIRE Facility
Background
Safety and functional considerations
Facility design
NICD BSL4 Facility
BSL4 laboratory work
BSL4 facility design
NICD Ebola Mobile Laboratory
Facility location and assessment
Laboratory setup

3. Achieving Containment
Strategies Using:
Inward directional airflow
Isolation dampers
Sealing service penetrations
Backflow prevention
Filtration
Standard Operating Procedures

4. Containment Barriers Primary barriers (safety equipment)
BSCs- all procedures
Personal Protective Equipment (PPE)
Isolators and Ventilated Racks
Secondary barriers (facilities)
Design parameters to protect people surrounding the laboratory and the community
Separate building or isolated zone
Special HVAC systems and controls
Lab
Ante Rm
Sterilizer
BSC
Primary Containment
Secondary Containment

5. Hvac systems and control
Room exhaust is through double HEPA filtration (BIBO safe-change housings)
Supply air is HEPA filtered
Supply air terminals (room) are have HEPA filters for the unlikely event of the lab going positive
Exhaust and supply air are interlocked, with fan redundancy
System response and control managed via a BMS

6. Architectural components
Stainless Steel Doors with Inflatable Door Seals
Surface Finishes – Epoxy based (3mm Floor, Walls and Ceilings Painted with Water-based Epoxy)

7. Sterilisation systems
Solid Waste Sterilisation – Autoclaves
Effluent Waste Sterilisation

8. NIOH aspire facility: Background
Investigation of airborne transmission of Mycobacterium tuberculosis (and other infectious agents within indoor environments);
Ultraviolet Germicidal Irradiation fixtures are implemented to inactivate airborne pathogens such as M.tuberculosis;
The objective to inactivate airborne infectious agents in the upper part of the room, while minimizing radiation exposure to persons in the lower part of the room;
The efficacy of commercially available germicidal lamps in South Africa is not known;
Testing will involve aerosolising M.tuberculosis in a room environment and to determine the ability of UVGI fixtures to inactivate M. tuberculosis in the test room;

9. aspire facility: Safety & functional considerations
Exposure to M.tuberculosis (avirulent/attenuated);
Environmental factors such as air velocity, air mixing, and relative humidity affect the efficacy of UVGI fixtures;
Moveable ceiling;
The aerosolised air sample collection and variable time intervals;

10. aspire facility: facility design
Biosafety Level 3 (BSL3) design;
Single-pass system with HEPA filtered exhaust;
HEPA filtered supply air;
Clean and dirty change rooms;
Negative pressure cascade;
Use of primary containment devices (Class II A2 BSC);
Use of PAPRs;

11. aspire facility: facility design

12. aspire facility: facility design

13. aspire facility: facility design

14. Nicd bsl4 facility: laboratory work
Diagnosis and Research
Development, Production and Validation of Diagnostic Reagents and Assays
Investigation of Emerging and Re-emerging Viral Diseases
Training
Developed Mobile Laboratory Unit (MLU) capacity

15. BSl4 facility: laboratory design

16. BSl4 facility: laboratory design

17. BSl4 facility: laboratory design

18. BSl4 facility: laboratory design

19. Nicd ebola mobile laboratory: sierra leone
The NICD MLU was deployed, through the WHO-GOARN recruitment mechanism, in Sierra Leone, Freetown on 17 August 2014 as part of WHO EVD outbreak response in West Africa.
Aug 24: Arrival of UN delegation, including Drs. Nabaro (UN) and Fukunaga (WHO) to visit the NICD Mobile Laboratory at Lakka Hospital, Freetown.  
Aug 25: President of the Republic of Sierra Leone Dr Ernest Bai Koroma and other governmental officials delegation visiting the NICD MLU at Lakka Hospital.
Aug 25: NICD MLU declared to be fully operational

20. mobile laboratory: Facility location & Assessment

21. mobile laboratory: Facility location & Assessment

22. mobile laboratory

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