1. Aim: How do certain factors, such as pH, temperature & enzyme shape affect enzyme action?
Q: Why would someone want to take these supplements?

2. Enzymes
All enzymes work best in optimal conditions

3. pH
The peak (top) of the line graph shows the best pH for this enzyme

4. Effect of pH on 2 Different Enzymes

5. Questions
Q1. In what kind of pH environment does enzyme pepsin function best?
Q2. In what kind of pH environment does enzyme salivary amylase function best?
Q3. What happens to the rate of enzyme activity when the pH is higher or lower than the optimal pH for a specific enzyme?

6. Temperature

7. Denaturation of Enzymes

8. Real World Denaturation

9. Summary
High temperature and strong acids and bases can change the enzyme’s shape
Result: Enzyme is denatured & cannot work
The rate of enzyme activity decreases above and below optimal (best) enzyme conditions

10. Video

11. Exit Slip
Answer the following questions on a piece of paper and turn answers in, before you leave class! Q1. List two factors that affect enzyme action. Q2. Explain denaturation. Q3. What site does a substrate molecule bind to, on an enzyme?