1. 97kDa Linear IgA Bullous Dermatosis Antigen Localizes in the Lamina Lucida Between the NC16A and Carboxyl Terminal Domains of the 180kDa Bullous Pemphigoid Antigen 
Akira Ishiko, Hiroshi Shimizu, Takuji Masunaga, Kim B. Yancey, George J. Giudice, John J. Zone, Takeji Nishikawa 
Journal of Investigative Dermatology 
Volume 111, Issue 1, Pages (July 1998)
DOI: /j x
Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Monoclonal antibodies against 97-LAD (97–1 and 97–2) bind to the lamina lucida of normal human skin in association with hemidesmosomes. Immunogold electron microscopy using cryo-ultrathin sections of normal human skin. Cryo-ultrathin sections were incubated with MoAb 97–1 or MoAb 97–2 followed by incubation with 5nm gold-conjugated anti-mouse immunoglobulins. Epitopes of MoAb 97–1 (a) and MoAb 97–2 (b) localized in the lamina lucida in close association with hemidesmosomes and no significant difference was observed between the two antibodies. Arrowhead, plasma membrane;*lamina lucida;arrow, lamina densa. Scale bars, 200nm.
Journal of Investigative Dermatology  , 93-96DOI: ( /j x)
Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Distribution histograms of the distance between the plasma membrane and the binding sites of MoAb 97–1 and anti-C terminus of BPAG2 polyclonal antibodies. The distances between more than 300 gold particles and the plasma membrane of basal keratinocytes were measured on electron micrographs of each case. (a) The distribution of the MoAb 97–1 binding site had a peak at 20nm and the mean distance from the plasma membrane was 28nm. (b) The distribution of anti-C terminus of the BPAG2 binding site had a peak at 40nm and the mean distance from the plasma membrane was 42nm.
Journal of Investigative Dermatology  , 93-96DOI: ( /j x)
Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
97-LAD localized between the NC16A domain and the C terminus of BPAG2. Double immunolabeling was done on cryo-ultrathin sections of normal human skin. (a) Epitope of MoAb 97–1 (5nm) localized at the lamina lucida beneath the NC16A domain of BPAG2 (15nm) that localized at the plasma membrane of the hemidesmosome complex. (b) Epitope of MoAb 97–1 (5nm) localized at the lamina lucida above the C-terminal domain of BPAG2 (15nm) that localized mainly at the lower lamina lucida. Scale bars, 200nm.
Journal of Investigative Dermatology  , 93-96DOI: ( /j x)
Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions